Measuring the peptides in individual organelles with mass spectrometry

Stanislav S. Rubakhin; Rebecca W. Garden; Robert R. Fuller; Jonathan V. Sweedler

doi:10.1038/72622

Measuring the peptides in individual organelles with mass spectrometry

Stanislav S. Rubakhin, Rebecca W. Garden, Robert R. Fuller, Jonathan V. Sweedler

Research output: Contribution to journal › Article › peer-review

Abstract

New sampling protocols combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allow the assay of single dense core vesicles. Understanding the packaging of vesicles is important as vesicles are the quanta of information for intercellular communication. Using vesicles from the exocrine atrial gland of Aplysia californica as the model, a wide range of bioactive peptides are detected within each vesicle. Although the expression of the egg-laying hormone gene family of type I atrial gland cells has been previously examined, chemical characterization of individual 1-2 μm diameter vesicles demonstrates that products from several genes are colocalized. The mass sensitivity of MALDI MS can be further improved to enable the analysis of even smaller subcellular organelles.

Original language	English (US)
Pages (from-to)	172-175
Number of pages	4
Journal	Nature Biotechnology
Volume	18
Issue number	2
DOIs	https://doi.org/10.1038/72622
State	Published - Feb 2000

Keywords

Aplysia californica
Atrial gland
Dense-core vesicle
Matix-assisted laser desorption/ionization
Peptide

ASJC Scopus subject areas

Microbiology

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10.1038/72622

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title = "Measuring the peptides in individual organelles with mass spectrometry",

abstract = "New sampling protocols combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allow the assay of single dense core vesicles. Understanding the packaging of vesicles is important as vesicles are the quanta of information for intercellular communication. Using vesicles from the exocrine atrial gland of Aplysia californica as the model, a wide range of bioactive peptides are detected within each vesicle. Although the expression of the egg-laying hormone gene family of type I atrial gland cells has been previously examined, chemical characterization of individual 1-2 μm diameter vesicles demonstrates that products from several genes are colocalized. The mass sensitivity of MALDI MS can be further improved to enable the analysis of even smaller subcellular organelles.",

keywords = "Aplysia californica, Atrial gland, Dense-core vesicle, Matix-assisted laser desorption/ionization, Peptide",

author = "Rubakhin, {Stanislav S.} and Garden, {Rebecca W.} and Fuller, {Robert R.} and Sweedler, {Jonathan V.}",

note = "Funding Information: We thank Gregg Nagle and Sherry Painter for insightful comments about atrial gland peptides, and we thank Christian Reilly for assistance with animal collection. This work is supported by the National Institutes of Health, the National Science Foundation, and the Dreyfus Foundation.",

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AU - Garden, Rebecca W.

AU - Fuller, Robert R.

AU - Sweedler, Jonathan V.

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N2 - New sampling protocols combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allow the assay of single dense core vesicles. Understanding the packaging of vesicles is important as vesicles are the quanta of information for intercellular communication. Using vesicles from the exocrine atrial gland of Aplysia californica as the model, a wide range of bioactive peptides are detected within each vesicle. Although the expression of the egg-laying hormone gene family of type I atrial gland cells has been previously examined, chemical characterization of individual 1-2 μm diameter vesicles demonstrates that products from several genes are colocalized. The mass sensitivity of MALDI MS can be further improved to enable the analysis of even smaller subcellular organelles.

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Measuring the peptides in individual organelles with mass spectrometry

Abstract

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ASJC Scopus subject areas

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