Measuring nerve growth factor in saliva by immunoassay: A cautionary note

Marla J. Matin, Daming Li, Jon Peterson, Marcus K. Taylor, Heidemarie K. Laurent, Todd Lucas, Steve J. Granger, Douglas A. Granger, Steve W. Granger

Research output: Contribution to journalArticlepeer-review


Nerve growth factor (NGF), a neurotrophin, modulates a diverse set of physiologic processes in the nervous, immune, and endocrine systems. Studies suggest that NGF can be measured in saliva (sNGF). Historically, the method for measuring sNGF involves the off-label use of an enzyme immunoassay designed for use with cell-culture supernatants/tissue extracts (Nam et al., 2007; Ruhl et al., 2004). In a series of experiments we reveal this measurement strategy is subject to non-specific interference by constituents present in oral fluids. We conclude that the measurement of sNGF by this assay is not optimal for use with oral fluid specimens.

Original languageEnglish (US)
Pages (from-to)235-237
Number of pages3
StatePublished - Jan 1 2016


  • Immunoassay
  • Interference
  • Methods
  • Nerve growth factor
  • Saliva

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Psychiatry and Mental health
  • Biological Psychiatry


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