Measuring diffusion in cell membranes by fluorescence correlation spectroscopy

Parijat Sengupta, J. Balaji, Sudipta Maiti

Research output: Contribution to journalArticlepeer-review

Abstract

Fluorescence Correlation Spectroscopy (FCS) can measure diffusion on the cell surface with unparalleled sensitivity. In appropriate situations, this can be the most sensitive and accurate method for measuring receptor interaction and oligomerization. Here we attempt to describe FCS in sufficient detail so that the reader is able to judge when there is a compelling reason to choose this technique, understand the basic theory behind it, construct a FCS spectrometer in the laboratory, and analyze the data to obtain a meaningful estimate of the physical parameters.

Original languageEnglish (US)
Pages (from-to)374-387
Number of pages14
JournalMethods
Volume27
Issue number4
DOIs
StatePublished - Oct 12 2002

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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