Measuring agreement and discord among hemagglutination inhibition assays against different ophidian paramyxovirus strains in the eastern massasauga (Sistrurus catenatus catenatus)

Research output: Contribution to journalArticle

Abstract

At present, the hemagglutination inhibition (HI) assay is the sole commercially available serologic method available to detect exposure to ophidian paramyxovirus (OPMV) in snakes. During 2006, 26 eastern massasaugas (Sistrurus catenates catenates) were collected, and blood was sampled to determine their OPMV status. Samples from each snake were divided into 3 aliquots and tested by using commercially available HI assays against the 4 OPMV isolates used in the 3 laboratories that offer the service. All snakes were tested for antibodies by using HI assays against the green tree python (GTP), San Lucan rattlesnake (SLR), and Aruba Island rattlesnake (AIR) isolates. Twenty-five snakes were tested for antibodies against the western diamondback rattlesnake (WDR) isolate. All samples tested against the GTP and SLR were positive (26/26), whereas 56% (14/25) of the WDR assays were positive, and none (0/ 26) of the AIR assays yielded a positive result. There was 100% agreement between the GTP and SLR assays, and complete disagreement between the SLR and AIR, as well as the GTP and AIR assays. Kappa statistics for the GTP-WDR, SLR-WDR, GTP-AIR, SLR-AIR, and WDR-AIR indicated that the assays had less than chance agreement. The results demonstrate that current HI assays are not reliable as a sole diagnostic assay in the eastern massasauga. Furthermore, HI assays need to be evaluated by using other parameters to determine OPMV exposure in eastern massasaugas.

Original languageEnglish (US)
Pages (from-to)358-361
Number of pages4
JournalJournal of Zoo and Wildlife Medicine
Volume39
Issue number3
DOIs
StatePublished - Sep 1 2008

Fingerprint

Sistrurus catenatus
Aruba
Crotalus
Respirovirus
hemagglutination inhibition test
Crotalus atrox
Hemagglutination
snakes
Boidae
assays
Islands
Netherlands
Snakes
Sistrurus
antibodies
Morelia viridis
statistics
sampling

Keywords

  • Eastern massasauga
  • Hemagglutination inhibition
  • Paramyxovirus
  • Sistrurus catenates
  • Snake

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

@article{168db35071de45c6a6ee124f615d640b,
title = "Measuring agreement and discord among hemagglutination inhibition assays against different ophidian paramyxovirus strains in the eastern massasauga (Sistrurus catenatus catenatus)",
abstract = "At present, the hemagglutination inhibition (HI) assay is the sole commercially available serologic method available to detect exposure to ophidian paramyxovirus (OPMV) in snakes. During 2006, 26 eastern massasaugas (Sistrurus catenates catenates) were collected, and blood was sampled to determine their OPMV status. Samples from each snake were divided into 3 aliquots and tested by using commercially available HI assays against the 4 OPMV isolates used in the 3 laboratories that offer the service. All snakes were tested for antibodies by using HI assays against the green tree python (GTP), San Lucan rattlesnake (SLR), and Aruba Island rattlesnake (AIR) isolates. Twenty-five snakes were tested for antibodies against the western diamondback rattlesnake (WDR) isolate. All samples tested against the GTP and SLR were positive (26/26), whereas 56{\%} (14/25) of the WDR assays were positive, and none (0/ 26) of the AIR assays yielded a positive result. There was 100{\%} agreement between the GTP and SLR assays, and complete disagreement between the SLR and AIR, as well as the GTP and AIR assays. Kappa statistics for the GTP-WDR, SLR-WDR, GTP-AIR, SLR-AIR, and WDR-AIR indicated that the assays had less than chance agreement. The results demonstrate that current HI assays are not reliable as a sole diagnostic assay in the eastern massasauga. Furthermore, HI assays need to be evaluated by using other parameters to determine OPMV exposure in eastern massasaugas.",
keywords = "Eastern massasauga, Hemagglutination inhibition, Paramyxovirus, Sistrurus catenates, Snake",
author = "Allender, {Matthew C} and Mitchell, {Mark A.} and Dreslik, {Michael Joseph} and Phillips, {Christopher A} and Beasley, {Val R.}",
year = "2008",
month = "9",
day = "1",
doi = "10.1638/2007-0111.1",
language = "English (US)",
volume = "39",
pages = "358--361",
journal = "Journal of Zoo and Wildlife Medicine",
issn = "1042-7260",
publisher = "American Association of Zoo Veterinarians",
number = "3",

}

TY - JOUR

T1 - Measuring agreement and discord among hemagglutination inhibition assays against different ophidian paramyxovirus strains in the eastern massasauga (Sistrurus catenatus catenatus)

AU - Allender, Matthew C

AU - Mitchell, Mark A.

AU - Dreslik, Michael Joseph

AU - Phillips, Christopher A

AU - Beasley, Val R.

PY - 2008/9/1

Y1 - 2008/9/1

N2 - At present, the hemagglutination inhibition (HI) assay is the sole commercially available serologic method available to detect exposure to ophidian paramyxovirus (OPMV) in snakes. During 2006, 26 eastern massasaugas (Sistrurus catenates catenates) were collected, and blood was sampled to determine their OPMV status. Samples from each snake were divided into 3 aliquots and tested by using commercially available HI assays against the 4 OPMV isolates used in the 3 laboratories that offer the service. All snakes were tested for antibodies by using HI assays against the green tree python (GTP), San Lucan rattlesnake (SLR), and Aruba Island rattlesnake (AIR) isolates. Twenty-five snakes were tested for antibodies against the western diamondback rattlesnake (WDR) isolate. All samples tested against the GTP and SLR were positive (26/26), whereas 56% (14/25) of the WDR assays were positive, and none (0/ 26) of the AIR assays yielded a positive result. There was 100% agreement between the GTP and SLR assays, and complete disagreement between the SLR and AIR, as well as the GTP and AIR assays. Kappa statistics for the GTP-WDR, SLR-WDR, GTP-AIR, SLR-AIR, and WDR-AIR indicated that the assays had less than chance agreement. The results demonstrate that current HI assays are not reliable as a sole diagnostic assay in the eastern massasauga. Furthermore, HI assays need to be evaluated by using other parameters to determine OPMV exposure in eastern massasaugas.

AB - At present, the hemagglutination inhibition (HI) assay is the sole commercially available serologic method available to detect exposure to ophidian paramyxovirus (OPMV) in snakes. During 2006, 26 eastern massasaugas (Sistrurus catenates catenates) were collected, and blood was sampled to determine their OPMV status. Samples from each snake were divided into 3 aliquots and tested by using commercially available HI assays against the 4 OPMV isolates used in the 3 laboratories that offer the service. All snakes were tested for antibodies by using HI assays against the green tree python (GTP), San Lucan rattlesnake (SLR), and Aruba Island rattlesnake (AIR) isolates. Twenty-five snakes were tested for antibodies against the western diamondback rattlesnake (WDR) isolate. All samples tested against the GTP and SLR were positive (26/26), whereas 56% (14/25) of the WDR assays were positive, and none (0/ 26) of the AIR assays yielded a positive result. There was 100% agreement between the GTP and SLR assays, and complete disagreement between the SLR and AIR, as well as the GTP and AIR assays. Kappa statistics for the GTP-WDR, SLR-WDR, GTP-AIR, SLR-AIR, and WDR-AIR indicated that the assays had less than chance agreement. The results demonstrate that current HI assays are not reliable as a sole diagnostic assay in the eastern massasauga. Furthermore, HI assays need to be evaluated by using other parameters to determine OPMV exposure in eastern massasaugas.

KW - Eastern massasauga

KW - Hemagglutination inhibition

KW - Paramyxovirus

KW - Sistrurus catenates

KW - Snake

UR - http://www.scopus.com/inward/record.url?scp=53549112768&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=53549112768&partnerID=8YFLogxK

U2 - 10.1638/2007-0111.1

DO - 10.1638/2007-0111.1

M3 - Article

VL - 39

SP - 358

EP - 361

JO - Journal of Zoo and Wildlife Medicine

JF - Journal of Zoo and Wildlife Medicine

SN - 1042-7260

IS - 3

ER -