Measurements of DNA lengths remaining in a viral capsid after osmotically suppressed partial ejection

Alex Evilevitch, James W. Gober, Martin Phillips, Charles M. Knobler, William M. Gelbart

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of external osmotic pressure on the extent of DNA ejection from bacteriophage-λ was recently investigated (Evilevitch et al., 2003). The total length of DNA ejected was measured via the 260-nm absorption by free nucleotides, after opening of the capsids in the presence of varying amounts of polyethylene glycol 8000 and DNase I. As a function of osmolyte concentration, this absorption was shown to decrease progressively, ultimately vanishing completely for a sufficiently high external osmotic pressure. In this work we report the results of both sedimentation and gel analysis of the length of DNA remaining inside the capsids, as a function of osmolyte concentration. It is confirmed in this way that the progressive inhibition of DNA ejection corresponds to partial ejection from all of the capsids.

Original languageEnglish (US)
Pages (from-to)751-756
Number of pages6
JournalBiophysical journal
Volume88
Issue number1
DOIs
StatePublished - Jan 2005

ASJC Scopus subject areas

  • Biophysics

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