Abstract
Ascorbate is one of the major antioxidant metabolites in plant tissues. This protocol describes a microplate-adapted colorimetric ascorbate assay, in which ferric ion is reduced by ascorbate to the ferrous ion. The ferrous ion reacts with α-ά-bipyridl to form a complex with characteristic absorbance at 525 nm. With the chemical reduction of any dehydroascorbate (DHA) in a sample, total ascorbate can be assayed using the α-ά-bipyridl method, and DHA can be estimated by subtracting the reduced portion from the total ascorbate pool. The assay is performed in microcentrifuge tubes and assessed in a 96-well plate reader. Reduced ascorbate, DHA and total ascorbate of at least 64 experimental samples can be analyzed easily in 1 d.
Original language | English (US) |
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Pages (from-to) | 871-874 |
Number of pages | 4 |
Journal | Nature Protocols |
Volume | 2 |
Issue number | 4 |
DOIs | |
State | Published - Apr 2007 |
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)