Measurement of local DNA reorganization on the picosecond and nanosecond time scales

Eric B. Brauns; Mihaela L. Madaras; Robert S. Coleman; Catherine J. Murphy; Mark A. Berg

doi:10.1021/ja992456q

Measurement of local DNA reorganization on the picosecond and nanosecond time scales

Eric B. Brauns, Mihaela L. Madaras, Robert S. Coleman, Catherine J. Murphy, Mark A. Berg

Research output: Contribution to journal › Article › peer-review

Abstract

Picosecond and nanosecond dynamics in the interior of DNA are observed for the first time as a dynamic Stokes shift in the fluorescence of a specially designed base-pair analogue. A recently synthesized coumarin C- riboside has been incorporated into an oligonucleotide in place of a normal purine·pyrimidine base pair. The dynamic Stokes shift of this probe occurs with components near 300 ps and 13 ns. These times are too long for solvent relaxation or simple vibrational motion of the DNA framework. They imply that the interior of DNA is a unique dynamic environment unlike either a fluid or a molecular crystal.

Original language	English (US)
Pages (from-to)	11644-11649
Number of pages	6
Journal	Journal of the American Chemical Society
Volume	121
Issue number	50
DOIs	https://doi.org/10.1021/ja992456q
State	Published - Dec 22 1999
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

Online availability

10.1021/ja992456q

Library availability

Discover UIUC Full Text

Cite this

@article{389e074a2dd640c0a58ee134363af2fa,

title = "Measurement of local DNA reorganization on the picosecond and nanosecond time scales",

abstract = "Picosecond and nanosecond dynamics in the interior of DNA are observed for the first time as a dynamic Stokes shift in the fluorescence of a specially designed base-pair analogue. A recently synthesized coumarin C- riboside has been incorporated into an oligonucleotide in place of a normal purine·pyrimidine base pair. The dynamic Stokes shift of this probe occurs with components near 300 ps and 13 ns. These times are too long for solvent relaxation or simple vibrational motion of the DNA framework. They imply that the interior of DNA is a unique dynamic environment unlike either a fluid or a molecular crystal.",

author = "Brauns, {Eric B.} and Madaras, {Mihaela L.} and Coleman, {Robert S.} and Murphy, {Catherine J.} and Berg, {Mark A.}",

year = "1999",

month = dec,

day = "22",

doi = "10.1021/ja992456q",

language = "English (US)",

volume = "121",

pages = "11644--11649",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "50",

}

TY - JOUR

T1 - Measurement of local DNA reorganization on the picosecond and nanosecond time scales

AU - Brauns, Eric B.

AU - Madaras, Mihaela L.

AU - Coleman, Robert S.

AU - Murphy, Catherine J.

AU - Berg, Mark A.

PY - 1999/12/22

Y1 - 1999/12/22

N2 - Picosecond and nanosecond dynamics in the interior of DNA are observed for the first time as a dynamic Stokes shift in the fluorescence of a specially designed base-pair analogue. A recently synthesized coumarin C- riboside has been incorporated into an oligonucleotide in place of a normal purine·pyrimidine base pair. The dynamic Stokes shift of this probe occurs with components near 300 ps and 13 ns. These times are too long for solvent relaxation or simple vibrational motion of the DNA framework. They imply that the interior of DNA is a unique dynamic environment unlike either a fluid or a molecular crystal.

AB - Picosecond and nanosecond dynamics in the interior of DNA are observed for the first time as a dynamic Stokes shift in the fluorescence of a specially designed base-pair analogue. A recently synthesized coumarin C- riboside has been incorporated into an oligonucleotide in place of a normal purine·pyrimidine base pair. The dynamic Stokes shift of this probe occurs with components near 300 ps and 13 ns. These times are too long for solvent relaxation or simple vibrational motion of the DNA framework. They imply that the interior of DNA is a unique dynamic environment unlike either a fluid or a molecular crystal.

UR - http://www.scopus.com/inward/record.url?scp=0033596294&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033596294&partnerID=8YFLogxK

U2 - 10.1021/ja992456q

DO - 10.1021/ja992456q

M3 - Article

AN - SCOPUS:0033596294

SN - 0002-7863

VL - 121

SP - 11644

EP - 11649

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 50

ER -

Measurement of local DNA reorganization on the picosecond and nanosecond time scales

Abstract

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this