Measurement of gene regulation in individual cells reveals rapid switching between promoter states

Leonardo A. Sepúlveda, Heng Xu, Jing Zhang, Mengyu Wang, Ido Golding

Research output: Contribution to journalArticle

Abstract

In vivo mapping of transcription-factor binding to the transcriptional output of the regulated gene is hindered by probabilistic promoter occupancy, the presence of multiple gene copies, and cell-To-cell variability.We demonstrate how to overcome these obstacles in the lysogeny maintenance promoter of bacteriophage lambda, PRM. We simultaneously measured the concentration of the lambda repressor CI and the number of messenger RNAs (mRNAs) from PRM in individual Escherichia coli cells, and used a theoretical model to identify the stochastic activity corresponding to different CI binding configurations. We found that switching between promoter configurations is faster than mRNA lifetime and that individual gene copies within the same cell act independently. The simultaneous quantification of transcription factor and promoter activity, followed by stochastic theoretical analysis, provides a tool that can be applied to other genetic circuits.

Original languageEnglish (US)
Pages (from-to)1218-1222
Number of pages5
JournalScience
Volume351
Issue number6278
DOIs
StatePublished - Mar 11 2016
Externally publishedYes

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Genes
Transcription Factors
Lysogeny
Bacteriophage lambda
Messenger RNA
Theoretical Models
Maintenance
Escherichia coli
phage repressor proteins

ASJC Scopus subject areas

  • General

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Measurement of gene regulation in individual cells reveals rapid switching between promoter states. / Sepúlveda, Leonardo A.; Xu, Heng; Zhang, Jing; Wang, Mengyu; Golding, Ido.

In: Science, Vol. 351, No. 6278, 11.03.2016, p. 1218-1222.

Research output: Contribution to journalArticle

Sepúlveda, Leonardo A. ; Xu, Heng ; Zhang, Jing ; Wang, Mengyu ; Golding, Ido. / Measurement of gene regulation in individual cells reveals rapid switching between promoter states. In: Science. 2016 ; Vol. 351, No. 6278. pp. 1218-1222.
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