@article{d02e7642039048398b554f435572c7d4,
title = "Measurement of Absolute Concentration at the Subcellular Scale",
abstract = "The concentration of a pharmaceutical drug or bioactive metabolite within the target organelle influences the effects elicited by the drug or metabolite. Although the relative concentrations of many compounds of interest within subcellular compartments have been measured, measurements of absolute concentrations in the organelle remain elusive. In this Perspective, we discuss a significant advance in using nano secondary ion mass spectrometry (nanoSIMS) to measure the absolute concentration of a 13C-labeled metabolite within secretory vesicles, as reported by Thomen et al. in the April issue of ACS Nano. ",
author = "Gorman, {Brittney L.} and Brunet, {Melanie A.} and Pham, {Susan N.} and Kraft, {Mary L.} and Kraft, {Mary L.} and Kraft, {Mary L.}",
note = "Funding Information: This material is based upon work supported by the National Science Foundation Graduate Research Fellowship Program under Grant No. DGE-1746047 (S.N.P.). Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation. This material is also based upon work supported by Grant No. T32 GM070421 from the Ruth L. Kirschstein Institutional National Research Service Award (M.B.). Its contents are solely the responsibility of the Chemistry-Biology Interface Training Program at UIUC and do not necessarily represent the official views of the NIH Research Training and Career Development. Support was also provided by the SURGE fellowship program (M.B.) and the Department of Chemical & Biomolecular Engineering at the University of Illinois, Urbana—Champaign. We gratefully acknowledge funds from the Chien Family Professorial Scholar. Publisher Copyright: {\textcopyright} 2020 American Chemical Society.",
year = "2020",
month = jun,
day = "23",
doi = "10.1021/acsnano.0c04285",
language = "English (US)",
volume = "14",
pages = "6414--6419",
journal = "ACS Nano",
issn = "1936-0851",
publisher = "American Chemical Society",
number = "6",
}