Measurement of Absolute Concentration at the Subcellular Scale

Brittney L. Gorman, Melanie A. Brunet, Susan N. Pham, Mary L. Kraft, Mary L. Kraft, Mary L. Kraft

Research output: Contribution to journalReview articlepeer-review


The concentration of a pharmaceutical drug or bioactive metabolite within the target organelle influences the effects elicited by the drug or metabolite. Although the relative concentrations of many compounds of interest within subcellular compartments have been measured, measurements of absolute concentrations in the organelle remain elusive. In this Perspective, we discuss a significant advance in using nano secondary ion mass spectrometry (nanoSIMS) to measure the absolute concentration of a 13C-labeled metabolite within secretory vesicles, as reported by Thomen et al. in the April issue of ACS Nano.

Original languageEnglish (US)
Pages (from-to)6414-6419
Number of pages6
JournalACS Nano
Issue number6
StatePublished - Jun 23 2020

ASJC Scopus subject areas

  • General Engineering
  • General Materials Science
  • General Physics and Astronomy


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