Mayflies in Ecotoxicity Testing: Methodological Needs and Knowledge Gaps.

Paul Sibley, Laurent Lagadic, Matt McCoole, Teresa Norberg-King, Ivo Roessink, David Soucek, Trudy Watson-Leung, Jeff Wirtz

Research output: Contribution to journalArticlepeer-review


In recognition of the growing interest in the application of mayflies in aquatic toxicity testing, a 1‐d virtual workshop was held in September 2018 to identify and discuss knowledge gaps that constrain advancements in the use of culture‐based and field‐collected mayfly species in toxicity testing. Twenty‐one experts from Europe and North America, representing industry, government, and academia, participated in the workshop. Prior to the workshop, a comprehensive literature review spanning the years 1933 to 2018 was conducted; this review covered a wide range of topics relating to mayfly species, including ecology, physiology, relative sensitivities, and toxicity test methods. The resulting articles (n  = ~3600) were reviewed and prioritized with respect to their relevance to the goals of the workshop, and an annotated bibliography was prepared incorporating articles that met these criteria (150 in total). The workshop comprised 4 key topic areas: ecology, culturing and maintenance, test methods and test designs, and a summary and path forward. The workshop focused on the mayfly species most commonly used in aquatic toxicity testing in Europe and North America: C. dipterum , N. triangulifer , and Hexagenia spp. The workshop identified and prioritized knowledge gaps that can serve as the basis for hypothesis‐driven research to assess the prospect for routine incorporation of mayflies in toxicity testing and the establishment of standardized test protocols. Several key questions were used to guide the workshop: Should emphasis be placed on further development of lab‐cultured species or on standardizing the use of field‐collected species? In tests emphasizing field‐collected species, how can holding, feeding, and testing conditions be improved to maximize organism health and successful use in testing? What criteria should be used in the selection of lab‐cultured or field‐collected test species? Should regulatory requirements play a role in determining species selection?
Original languageEnglish (US)
Pages (from-to)292-293
Number of pages2
JournalIntegrated environmental assessment and management
Issue number2
StatePublished - Mar 1 2020


  • INHS

ASJC Scopus subject areas

  • Geography, Planning and Development
  • Environmental Science(all)


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