Matrix metalloproteinase activation predicts amelioration of remodeling after dietary modification in injured arteries

Sina Tavakoli, Mahmoud Razavian, Jiasheng Zhang, Lei Nie, Ravi Marfatia, Lawrence W. Dobrucki, Albert J. Sinusas, Simon Robinson, D. Scott Edwards, Mehran M. Sadeghi

Research output: Contribution to journalArticlepeer-review

Abstract

Objective- To establish and validate early noninvasive imaging of matrix metalloproteinase (MMP) activation for monitoring the progression of vascular remodeling and response to dietary modification. Methods and results- Apolipoprotein E mice that were fed a high-fat diet underwent left common carotid artery wire injury. One week after surgery, a group of animals were withdrawn from the high-fat diet. The other group of animals continued that diet throughout the study. Micro single-photon emission computed tomographic (microSPECT)/CT imaging with RP805 (a Tc-labeled tracer targeting activated MMPs) was repeatedly performed at 2 and 4 weeks after surgery. Histological analysis at 4 weeks showed significant left carotid neointima formation, monocyte/macrophage infiltration, and upregulation of several MMPs, which were ameliorated by withdrawal from the high-fat diet. In vivo microSPECT/CT images visualized significant RP805 uptake, reflecting MMP activation, in the injured carotid arteries. MMP activation was reduced as early as 1 week after withdrawal from the high-fat diet and significantly correlated with neointimal area at 4 weeks after surgery. Conclusion- MMP activation predicts the progression of vascular remodeling and can track the effect of dietary modification after vascular injury.

Original languageEnglish (US)
Pages (from-to)102-109
Number of pages8
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume31
Issue number1
DOIs
StatePublished - Jan 2011
Externally publishedYes

Keywords

  • carotid arteries
  • imaging agents
  • metalloproteinases
  • vascular biology

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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