Maternal Plane of Nutrition during Late-Gestation and Weaning Age Alter Steer Calf Longissimus Muscle Adipogenic MicroRNA and Target Gene Expression

Sonia J. Moisá, Daniel W. Shike, Lindsay Shoup, Juan J. Loor

Research output: Contribution to journalArticle

Abstract

The main objective was to evaluate if different planes of maternal nutrition during late gestation and weaning age alter microRNA (miRNA) and target gene expression in offspring longissimus muscle (LM). Early (EW) and normal weaned (NW) Angus × Simmental calves (n = 30) born to cows that were grazing endophyte-infected tall fescue and red clover pastures with no supplement [low plane of nutrition (LPN)], or supplemented with 2.3 and 9.1 kg of dried distiller's grains with solubles and soy hulls [medium and high plane of nutrition (MPN, HPN), respectively] during the last 105 ± 11 days of gestation were used. Biopsies of LM were harvested at 78 (early weaning), 187 (normal weaning) and 354 days of age. Results indicate a role of pro-adipogenic miRNA in the control of adipogenesis in LM of NW-MPN steers between 78 and 187 days of age through upregulation of (1) miR-103 which inhibits CAV1, a protein that destabilizes INSR and leads to insulin resistance; (2) miR-143 which inhibits DLK1, a protein that inhibits adipocyte differentiation; and (3) miR-21 which impairs TGFBR2-induced inhibition of adipocyte differentiation. Among the studied anti-adipogenic miRNA, cow plane of nutrition resulted in downregulation of miR-34a expression in MPN steers compared with HPN and LPN at 78 days of age. Data for miR-34a provided a potential sign of epigenetic regulation of LM in beef offspring due to the cow plane of nutrition during late gestation.

Original languageEnglish (US)
Pages (from-to)123-138
Number of pages16
JournalLipids
Volume51
Issue number1
DOIs
StatePublished - Jan 1 2016

Fingerprint

Nutrition
Weaning
MicroRNAs
Gene expression
Muscle
Mothers
Gene Expression
Pregnancy
Muscles
Adipocytes
Endophytes
Trifolium
Adipogenesis
Epigenomics
Insulin Resistance
Beef
Proteins
Up-Regulation
Down-Regulation
Biopsy

Keywords

  • Epigenetics
  • Fetal programming
  • Marbling
  • Nutrition

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

Cite this

Maternal Plane of Nutrition during Late-Gestation and Weaning Age Alter Steer Calf Longissimus Muscle Adipogenic MicroRNA and Target Gene Expression. / Moisá, Sonia J.; Shike, Daniel W.; Shoup, Lindsay; Loor, Juan J.

In: Lipids, Vol. 51, No. 1, 01.01.2016, p. 123-138.

Research output: Contribution to journalArticle

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