Maternal Low-Fat Diet Programs the Hepatic Epigenome despite Exposure to an Obesogenic Postnatal Diet

Laura Moody, Justin Shao, Hong Chen, Yuan-Xiang Pan

Research output: Contribution to journalArticle

Abstract

Obesity and metabolic disease present a danger to long-term health outcomes. It has been hypothesized that epigenetic marks established during early life might program individuals and have either beneficial or harmful consequences later in life. In the present study, we examined whether maternal diet alters DNA methylation and whether such modifications persist after an obesogenic postnatal dietary challenge. During gestation and lactation, male Sprague-Dawley rats were exposed to either a high-fat diet (HF; n = 10) or low-fat diet (LF; n = 10). After weaning, all animals were fed a HF diet for an additional nine weeks. There were no differences observed in food intake or body weight between groups. Hepatic DNA methylation was quantified using both methylated DNA immunoprecipitation sequencing (MeDIP-seq) and methylation-sensitive restriction enzyme sequencing (MRE-seq). Overall, 1419 differentially methylated regions (DMRs) were identified. DMRs tended to be located in CpG shores and were enriched for genes involved in metabolism and cancer. Gene expression was measured for 31 genes in these pathways. Map3k5 and Igf1r were confirmed to be differentially expressed. Finally, we attempted to quantify the functional relevance of intergenic DMRs. Using chromatin contact data, we saw that conserved DMRs were topologically associated with metabolism genes, which were associated with differential expression of Adh5, Enox1, and Pik3c3. We show that although maternal dietary fat is unable to reverse offspring weight gain in response to a postnatal obesogenic diet, early life diet does program the hepatic methylome. Epigenetic alterations occur primarily in metabolic and cancer pathways and are associated with altered gene expression, but it is unclear whether they bear consequence later in life.

Original languageEnglish (US)
Article number2075
JournalNutrients
Volume11
Issue number9
DOIs
StatePublished - Sep 3 2019

Fingerprint

Fat-Restricted Diet
low fat diet
DNA Methylation
Mothers
DNA methylation
Diet
epigenetics
liver
Liver
Epigenomics
diet
gene expression
maternal nutrition
neoplasms
metabolism
genes
metabolic diseases
high fat diet
Genes
Gene Expression

Keywords

  • DO Had
  • developmental programming
  • early life nutrition
  • gene-environment interactions
  • hepatic methylome

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

Cite this

Maternal Low-Fat Diet Programs the Hepatic Epigenome despite Exposure to an Obesogenic Postnatal Diet. / Moody, Laura; Shao, Justin; Chen, Hong; Pan, Yuan-Xiang.

In: Nutrients, Vol. 11, No. 9, 2075, 03.09.2019.

Research output: Contribution to journalArticle

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