TY - JOUR
T1 - Mate (Ilex paraguariensis St. Hilaire) saponins induce caspase-3-dependent apoptosis in human colon cancer cells in vitro
AU - Puangpraphant, Sirima
AU - Berhow, Mark A.
AU - de Mejia, Elvira Gonzalez
PY - 2011/4/15
Y1 - 2011/4/15
N2 - Saponins are naturally occurring metabolites associated with several health benefits. The objective was to quantify and purify saponins from mate dry leaves, and to assess their anti inflammatory and apoptotic mechanisms in human colon cancer cells in vitro. Matesaponins were extracted with methanol from dry leaves, partially purified and quantified. Leaves contained 10-15mg/g dry weight total saponins, predominantly matesaponins 1 and 2. HPLC and LC/ESI-MS-MS identified saponins in six preparative chromatographic fractions (A, B, C, D, E, and F). Major matesaponins were identified as 1 [M-H]-=911 and 2 [M-H]-=1057, with trace amounts of 3 [M-H]-=1073, 4 [M-H]-=1219, and 5 [M-H]-=1383. Fractions D, E, and F significantly inhibited iNOS (IC35=36.3, 29.5, 43.7μM), PGE2 (IC35=23.1, 22.3, 11.7μM) and COX-2 (IC35=45.7, 32.4, 17.0μM). Fraction F reduced nuclear translocation of nuclear factor-κB subunits p50 (49.8%) and p65 (49.0%) and induced apoptosis through suppression of Bcl-2 and increased Bax protein expressions and activated caspase-3 activity. Saponins in leaves of mate prevent inflammation and colon cancer in vitro.
AB - Saponins are naturally occurring metabolites associated with several health benefits. The objective was to quantify and purify saponins from mate dry leaves, and to assess their anti inflammatory and apoptotic mechanisms in human colon cancer cells in vitro. Matesaponins were extracted with methanol from dry leaves, partially purified and quantified. Leaves contained 10-15mg/g dry weight total saponins, predominantly matesaponins 1 and 2. HPLC and LC/ESI-MS-MS identified saponins in six preparative chromatographic fractions (A, B, C, D, E, and F). Major matesaponins were identified as 1 [M-H]-=911 and 2 [M-H]-=1057, with trace amounts of 3 [M-H]-=1073, 4 [M-H]-=1219, and 5 [M-H]-=1383. Fractions D, E, and F significantly inhibited iNOS (IC35=36.3, 29.5, 43.7μM), PGE2 (IC35=23.1, 22.3, 11.7μM) and COX-2 (IC35=45.7, 32.4, 17.0μM). Fraction F reduced nuclear translocation of nuclear factor-κB subunits p50 (49.8%) and p65 (49.0%) and induced apoptosis through suppression of Bcl-2 and increased Bax protein expressions and activated caspase-3 activity. Saponins in leaves of mate prevent inflammation and colon cancer in vitro.
KW - Apoptosis
KW - Colon cancer
KW - Inflammation
KW - Saponins
KW - Yerba Mate
UR - http://www.scopus.com/inward/record.url?scp=78649315870&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78649315870&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2010.10.023
DO - 10.1016/j.foodchem.2010.10.023
M3 - Article
AN - SCOPUS:78649315870
SN - 0308-8146
VL - 125
SP - 1171
EP - 1178
JO - Food chemistry
JF - Food chemistry
IS - 4
ER -