Over the past two decades, mass spectrometry has revolutionized protein analysis, developing as a mainstream neuroproteomic technology well suited to studying protein structure and function. This article overviews mass spectrometric capabilities, instrumentation, and approaches used to detect, sequence, and identify proteins in biological samples without the use of specific molecular probes and labels, often directly in brain tissue and cells. Strategies for mass spectrometric determination of the absolute and relative amounts of selected proteins in a sample are described. Application of mass spectrometry to the analysis of structural variability, posttranslational modifications, protein interactions, and biomarker discovery are highlighted.

Original languageEnglish (US)
Title of host publicationEncyclopedia of Neuroscience
PublisherElsevier Ltd
Number of pages7
ISBN (Print)9780080450469
StatePublished - 2009


  • Biomarker discovery
  • Ionization techniques
  • Mass analyzers
  • Neuroproteomics
  • Peptide mass fingerprinting
  • Posttranslational modifications
  • Protein complexes
  • Protein identification
  • Quantitation
  • Tandem mass spectrometry
  • Top-down sequencing

ASJC Scopus subject areas

  • General Neuroscience


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