Abstract

Mass spectrometry imaging and profiling of individual cells and subcellular structures provide unique analytical capabilities for biological and biomedical research, including determination of the biochemical heterogeneity of cellular populations and intracellular localization of pharmaceuticals. Two mass spectrometry technologies-secondary ion mass spectrometry (SIMS) and matrix assisted laser desorption/ionization mass spectrometry (MALDI MS)-are most often used in micro-bioanalytical investigations. Recent advances in ion probe technologies have increased the dynamic range and sensitivity of analyte detection by SIMS, allowing two- and three-dimensional localization of analytes in a variety of cells. SIMS operating in the mass spectrometry imaging (MSI) mode can routinely reach spatial resolutions at the submicron level; therefore, it is frequently used in studies of the chemical composition of subcellular structures. MALDI MS offers a large mass range and high sensitivity of analyte detection. It has been successfully applied in a variety of single-cell and organelle profiling studies. Innovative instrumentation such as scanning microprobe MALDI and mass microscope spectrometers enables new subcellular MSI measurements. Other approaches for MS-based chemical imaging and profiling include those based on near-field laser ablation and inductively-coupled plasma MS analysis, which offer complementary capabilities for subcellular chemical imaging and profiling.This article is part of a Special Issue entitled: Imaging Mass Spectrometry: A User's Guide to a New Technique for Biological and Biomedical Research.

Original languageEnglish (US)
Pages (from-to)5036-5051
Number of pages16
JournalJournal of Proteomics
Volume75
Issue number16
DOIs
StatePublished - Aug 30 2012

Fingerprint

Mass spectrometry
Secondary Ion Mass Spectrometry
Mass Spectrometry
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Imaging techniques
Secondary ion mass spectrometry
Biomedical Research
Technology
Ionization
Desorption
Laser Therapy
Population Characteristics
Organelles
Lasers
Inductively coupled plasma
Laser ablation
Ions
Spectrometers
Microscopes
Scanning

Keywords

  • Elemental imaging
  • Mass cytometry
  • Matrix assisted laser desorption/ionization
  • Secondary ion mass spectrometry
  • Subcellular profiling
  • Tissue imaging

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Mass spectrometry imaging and profiling of single cells. / Lanni, Eric J.; Rubakhin, Stanislav; Sweedler, Jonathan V.

In: Journal of Proteomics, Vol. 75, No. 16, 30.08.2012, p. 5036-5051.

Research output: Contribution to journalReview article

@article{ddf8dae82e9747edbf010de8ed99ded9,
title = "Mass spectrometry imaging and profiling of single cells",
abstract = "Mass spectrometry imaging and profiling of individual cells and subcellular structures provide unique analytical capabilities for biological and biomedical research, including determination of the biochemical heterogeneity of cellular populations and intracellular localization of pharmaceuticals. Two mass spectrometry technologies-secondary ion mass spectrometry (SIMS) and matrix assisted laser desorption/ionization mass spectrometry (MALDI MS)-are most often used in micro-bioanalytical investigations. Recent advances in ion probe technologies have increased the dynamic range and sensitivity of analyte detection by SIMS, allowing two- and three-dimensional localization of analytes in a variety of cells. SIMS operating in the mass spectrometry imaging (MSI) mode can routinely reach spatial resolutions at the submicron level; therefore, it is frequently used in studies of the chemical composition of subcellular structures. MALDI MS offers a large mass range and high sensitivity of analyte detection. It has been successfully applied in a variety of single-cell and organelle profiling studies. Innovative instrumentation such as scanning microprobe MALDI and mass microscope spectrometers enables new subcellular MSI measurements. Other approaches for MS-based chemical imaging and profiling include those based on near-field laser ablation and inductively-coupled plasma MS analysis, which offer complementary capabilities for subcellular chemical imaging and profiling.This article is part of a Special Issue entitled: Imaging Mass Spectrometry: A User's Guide to a New Technique for Biological and Biomedical Research.",
keywords = "Elemental imaging, Mass cytometry, Matrix assisted laser desorption/ionization, Secondary ion mass spectrometry, Subcellular profiling, Tissue imaging",
author = "Lanni, {Eric J.} and Stanislav Rubakhin and Sweedler, {Jonathan V}",
year = "2012",
month = "8",
day = "30",
doi = "10.1016/j.jprot.2012.03.017",
language = "English (US)",
volume = "75",
pages = "5036--5051",
journal = "Journal of Proteomics",
issn = "1874-3919",
publisher = "Elsevier",
number = "16",

}

TY - JOUR

T1 - Mass spectrometry imaging and profiling of single cells

AU - Lanni, Eric J.

AU - Rubakhin, Stanislav

AU - Sweedler, Jonathan V

PY - 2012/8/30

Y1 - 2012/8/30

N2 - Mass spectrometry imaging and profiling of individual cells and subcellular structures provide unique analytical capabilities for biological and biomedical research, including determination of the biochemical heterogeneity of cellular populations and intracellular localization of pharmaceuticals. Two mass spectrometry technologies-secondary ion mass spectrometry (SIMS) and matrix assisted laser desorption/ionization mass spectrometry (MALDI MS)-are most often used in micro-bioanalytical investigations. Recent advances in ion probe technologies have increased the dynamic range and sensitivity of analyte detection by SIMS, allowing two- and three-dimensional localization of analytes in a variety of cells. SIMS operating in the mass spectrometry imaging (MSI) mode can routinely reach spatial resolutions at the submicron level; therefore, it is frequently used in studies of the chemical composition of subcellular structures. MALDI MS offers a large mass range and high sensitivity of analyte detection. It has been successfully applied in a variety of single-cell and organelle profiling studies. Innovative instrumentation such as scanning microprobe MALDI and mass microscope spectrometers enables new subcellular MSI measurements. Other approaches for MS-based chemical imaging and profiling include those based on near-field laser ablation and inductively-coupled plasma MS analysis, which offer complementary capabilities for subcellular chemical imaging and profiling.This article is part of a Special Issue entitled: Imaging Mass Spectrometry: A User's Guide to a New Technique for Biological and Biomedical Research.

AB - Mass spectrometry imaging and profiling of individual cells and subcellular structures provide unique analytical capabilities for biological and biomedical research, including determination of the biochemical heterogeneity of cellular populations and intracellular localization of pharmaceuticals. Two mass spectrometry technologies-secondary ion mass spectrometry (SIMS) and matrix assisted laser desorption/ionization mass spectrometry (MALDI MS)-are most often used in micro-bioanalytical investigations. Recent advances in ion probe technologies have increased the dynamic range and sensitivity of analyte detection by SIMS, allowing two- and three-dimensional localization of analytes in a variety of cells. SIMS operating in the mass spectrometry imaging (MSI) mode can routinely reach spatial resolutions at the submicron level; therefore, it is frequently used in studies of the chemical composition of subcellular structures. MALDI MS offers a large mass range and high sensitivity of analyte detection. It has been successfully applied in a variety of single-cell and organelle profiling studies. Innovative instrumentation such as scanning microprobe MALDI and mass microscope spectrometers enables new subcellular MSI measurements. Other approaches for MS-based chemical imaging and profiling include those based on near-field laser ablation and inductively-coupled plasma MS analysis, which offer complementary capabilities for subcellular chemical imaging and profiling.This article is part of a Special Issue entitled: Imaging Mass Spectrometry: A User's Guide to a New Technique for Biological and Biomedical Research.

KW - Elemental imaging

KW - Mass cytometry

KW - Matrix assisted laser desorption/ionization

KW - Secondary ion mass spectrometry

KW - Subcellular profiling

KW - Tissue imaging

UR - http://www.scopus.com/inward/record.url?scp=84865179242&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84865179242&partnerID=8YFLogxK

U2 - 10.1016/j.jprot.2012.03.017

DO - 10.1016/j.jprot.2012.03.017

M3 - Review article

C2 - 22498881

AN - SCOPUS:84865179242

VL - 75

SP - 5036

EP - 5051

JO - Journal of Proteomics

JF - Journal of Proteomics

SN - 1874-3919

IS - 16

ER -