Abstract
To meet the ever-increasing need for high-throughput screening in metabolic engineering, information-rich, fast screening methods are needed. Mass spectrometry (MS) provides an efficient and general approach for metabolite screening and offers the capability of characterizing a broad range of analytes in a label-free manner, but often requires a range of sample clean-up and extraction steps. Liquid extraction surface analysis (LESA) coupled MS is an image-guided MS surface analysis approach that directly samples and introduces metabolites from a surface to MS. Here, we combined the advantages of LESA-MS and an acoustic liquid handler with stable isotope-labeled internal standards. This approach provides absolute quantitation of target chemicals from liquid culture-dried droplets and enables high-throughput quantitative screening for microbial metabolites. In this study, LESA-MS was successfully applied to quantify several different metabolites (itaconic acid, triacetic acid lactone, and palmitic acid) from different yeast strains in different mediums, demonstrating its versatility, accuracy, and efficiency across a range of microbial engineering applications.
Original language | English (US) |
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Pages (from-to) | 4067-4076 |
Number of pages | 10 |
Journal | Analytical Chemistry |
Volume | 95 |
Issue number | 8 |
DOIs | |
State | Published - Feb 28 2023 |
ASJC Scopus subject areas
- Analytical Chemistry
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Data for Mass Spectrometry based High-Throughput Quantification of Bioproducts in Liquid Culture
Zhou, S. (Creator) & Sweedler, J. V. (Creator), University of Illinois Urbana-Champaign, Mar 6 2023
DOI: 10.13012/B2IDB-5344291_V1
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