Mass spectrometric survey of interganglionically transported peptides in Aplysia

Lingjun Li; Tatiana P. Moroz; Rebecca W. Garden; Philip D. Floyd; Klaudiusz R. Weiss; Jonathan V. Sweedler

doi:10.1016/S0196-9781(98)00094-1

Mass spectrometric survey of interganglionically transported peptides in Aplysia

Lingjun Li, Tatiana P. Moroz, Rebecca W. Garden, Philip D. Floyd, Klaudiusz R. Weiss, Jonathan V. Sweedler

Research output: Contribution to journal › Article › peer-review

Abstract

The major ganglionic connectives in Aplysia are assayed to determine putative neuropeptides. Matrix-assisted laser desorption/ionization mass spectrometry allows direct measurement of peptides in a nerve. Many previously characterized peptides are observed, including APGWamide, buccalins, small cardioactive peptides, and egg-laying hormone. Several unreported peptides are detected in specific nerves, suggesting they may have important physiological roles. Furthermore, novel processing products of the L5-67 precursor peptide and the APGWamide/cerebral peptide 1 prohormone are strongly suggested, and their interganglionic transport demonstrated.

Original language	English (US)
Pages (from-to)	1425-1433
Number of pages	9
Journal	Peptides
Volume	19
Issue number	8
DOIs	https://doi.org/10.1016/S0196-9781(98)00094-1
State	Published - 1998

Keywords

Aplysia californica
Cerebral peptide I (CPI)
Interganglionic transport
LUQIN
Mass spectrometry (MS)
Matrix-assisted laser desorption/ionization (MALDI)
Neuropeptide
Peptide processing

ASJC Scopus subject areas

Biochemistry
Endocrinology
Physiology
Cellular and Molecular Neuroscience

Online availability

10.1016/S0196-9781(98)00094-1

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@article{657e6db10a0044738ca9107556a0f847,

title = "Mass spectrometric survey of interganglionically transported peptides in Aplysia",

abstract = "The major ganglionic connectives in Aplysia are assayed to determine putative neuropeptides. Matrix-assisted laser desorption/ionization mass spectrometry allows direct measurement of peptides in a nerve. Many previously characterized peptides are observed, including APGWamide, buccalins, small cardioactive peptides, and egg-laying hormone. Several unreported peptides are detected in specific nerves, suggesting they may have important physiological roles. Furthermore, novel processing products of the L5-67 precursor peptide and the APGWamide/cerebral peptide 1 prohormone are strongly suggested, and their interganglionic transport demonstrated.",

keywords = "Aplysia californica, Cerebral peptide I (CPI), Interganglionic transport, LUQIN, Mass spectrometry (MS), Matrix-assisted laser desorption/ionization (MALDI), Neuropeptide, Peptide processing",

author = "Lingjun Li and Moroz, {Tatiana P.} and Garden, {Rebecca W.} and Floyd, {Philip D.} and Weiss, {Klaudiusz R.} and Sweedler, {Jonathan V.}",

note = "Funding Information: This study is supported by National Institutes of Health (NIH) Grant NS31609 and National Science Foundation Grant CHE 96–22663 (to J. V. S.). Aplysia californica were partially provided by the National Resource for Aplysia at the University of Miami under NIH National Center for Research Resources Grant RR10294. R. W. G. acknowledges support from an Eastman Fellowship in Analytical Chemistry and Polymer characterization.",

year = "1998",

doi = "10.1016/S0196-9781(98)00094-1",

language = "English (US)",

volume = "19",

pages = "1425--1433",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "8",

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TY - JOUR

T1 - Mass spectrometric survey of interganglionically transported peptides in Aplysia

AU - Li, Lingjun

AU - Moroz, Tatiana P.

AU - Garden, Rebecca W.

AU - Floyd, Philip D.

AU - Weiss, Klaudiusz R.

AU - Sweedler, Jonathan V.

N1 - Funding Information: This study is supported by National Institutes of Health (NIH) Grant NS31609 and National Science Foundation Grant CHE 96–22663 (to J. V. S.). Aplysia californica were partially provided by the National Resource for Aplysia at the University of Miami under NIH National Center for Research Resources Grant RR10294. R. W. G. acknowledges support from an Eastman Fellowship in Analytical Chemistry and Polymer characterization.

PY - 1998

Y1 - 1998

N2 - The major ganglionic connectives in Aplysia are assayed to determine putative neuropeptides. Matrix-assisted laser desorption/ionization mass spectrometry allows direct measurement of peptides in a nerve. Many previously characterized peptides are observed, including APGWamide, buccalins, small cardioactive peptides, and egg-laying hormone. Several unreported peptides are detected in specific nerves, suggesting they may have important physiological roles. Furthermore, novel processing products of the L5-67 precursor peptide and the APGWamide/cerebral peptide 1 prohormone are strongly suggested, and their interganglionic transport demonstrated.

AB - The major ganglionic connectives in Aplysia are assayed to determine putative neuropeptides. Matrix-assisted laser desorption/ionization mass spectrometry allows direct measurement of peptides in a nerve. Many previously characterized peptides are observed, including APGWamide, buccalins, small cardioactive peptides, and egg-laying hormone. Several unreported peptides are detected in specific nerves, suggesting they may have important physiological roles. Furthermore, novel processing products of the L5-67 precursor peptide and the APGWamide/cerebral peptide 1 prohormone are strongly suggested, and their interganglionic transport demonstrated.

KW - Aplysia californica

KW - Cerebral peptide I (CPI)

KW - Interganglionic transport

KW - LUQIN

KW - Mass spectrometry (MS)

KW - Matrix-assisted laser desorption/ionization (MALDI)

KW - Neuropeptide

KW - Peptide processing

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M3 - Article

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AN - SCOPUS:0031758717

SN - 0196-9781

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JO - Peptides

JF - Peptides

IS - 8

ER -

Mass spectrometric survey of interganglionically transported peptides in Aplysia

Abstract

Keywords

ASJC Scopus subject areas

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