Marek's disease virus (MDV) ubiquitin-specific protease (USP) performs critical functions beyond its enzymatic activity during virus replication

Inĉs B. Veiga, Keith W. Jarosinski, Benedikt B. Kaufer, Nikolaus Osterrieder

Research output: Contribution to journalArticle

Abstract

Marek's disease virus (MDV) encodes an ubiquitin-specific protease (USP) within its UL36 gene. USP is highly conserved among herpesviruses and was shown to be important for MDV replication and pathogenesis in MDV's natural host, the chicken. To further investigate the role of MDV USP, several recombinant (r) MDVs were generated and their in vitro phenotypes were evaluated using plaque size and growth kinetics assays. We discovered that the N-terminus of pUL36 is essential for MDV replication and could not be complemented by ectopic expression of MDV USP. In addition, we demonstrated that the region located between the conserved glutamine (Q85) and leucine (L106) residues comprising the active site cysteine (C98) is also essential for MDV replication. Based on the analyses of the rMDVs generated here, we concluded that MDV USP likely contributes to the structure and/or stability of pUL36 and affects replication and oncogenesis of MDV beyond its enzymatic activity.

Original languageEnglish (US)
Pages (from-to)110-117
Number of pages8
JournalVirology
Volume437
Issue number2
DOIs
StatePublished - Mar 15 2013
Externally publishedYes

Keywords

  • Chicken
  • Deubiquitinase (DUB)
  • Marek's disease virus
  • Pathogenesis
  • Ubiquitin-specific protease (USP)
  • Viral replication

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Marek's disease virus (MDV) ubiquitin-specific protease (USP) performs critical functions beyond its enzymatic activity during virus replication'. Together they form a unique fingerprint.

  • Cite this