@article{170466073d2d4af0adb0452ded207d34,
title = "Mapping and confirmation of two genes conferring resistance to soybean rust (Phakopsora pachyrhizi) in the soybean line UG-5 (Glycine max)",
abstract = "Development of durable resistance to soybean rust (SBR) is challenging due to the pathogenic diversity of Phakopsora pachyrhizi populations. The objective of this research was to investigate and confirm the genomic locations of Rpp genes in the Ugandan line UG-5 that confer resistance to different SBR pathotypes. Bulked segregant analysis revealed two genomic regions associated with resistance in a cross with rust-susceptible 'Williams 82'. Composite interval mapping in the F2 and F2:3 populations had a LOD score of 48.7 in a region 0.38 cM away from the estimated location of the Rpp1 locus on chromosome (Chr.) 18. An approximately 23-Kbp interval spanning the Rpp1 locus was flanked by SNP markers ss715632313 and ss715632318. Another interval was identified at the Rpp3 locus on Chr. 6 between markers Satt100 and ss715594488 (2.4 cM) in the F2 population and between Satt100 and ss715594874 (4.3 cM) in the F2:3 population, with a maximum LOD score of 25.6. UG-5 was thus confirmed to have SBR resistance genes at the Rpp1 and Rpp3 loci that can be pyramided into other elite cultivars.",
keywords = "Phakopsora pachyrhizi, Rpp genes, bulked segregant analysis, rust resistance, soybean, soybean rust",
author = "Chandra Paul and Hartman, \{Glen L.\} and Diers, \{Brian W.\} and Walker, \{David R.\}",
note = "Funding was provided by the U.S. Agency for International Development as a linkage grant to the International Institute for Tropical Agriculture (IITA) and the USDA‐ARS in collaboration with the Soybean Pathology Laboratory, Department of Crop Sciences at the University of Illinois in Urbana‐Champaign. The authors would like to thank Dr. Zachary R. King, formerly at the University of Georgia, for his constructive discussion and recommendations on the KASP SNP genotyping approach; and Marcus O. Olatoye, Postdoctoral Research Associate, Department of Crop Sciences, University of Illinois at Urbana‐Champaign, for assistance with MapChart. Funding was provided by the U.S. Agency for International Development as a linkage grant to the International Institute for Tropical Agriculture (IITA) and the USDA-ARS in collaboration with the Soybean Pathology Laboratory, Department of Crop Sciences at the University of Illinois in Urbana-Champaign. The authors would like to thank Dr. Zachary R. King, formerly at the University of Georgia, for his constructive discussion and recommendations on the KASP SNP genotyping approach; and Marcus O. Olatoye, Postdoctoral Research Associate, Department of Crop Sciences, University of Illinois at Urbana-Champaign, for assistance with MapChart.",
year = "2020",
month = oct,
day = "1",
doi = "10.1111/pbr.12854",
language = "English (US)",
volume = "139",
pages = "932--942",
journal = "Plant Breeding",
issn = "0179-9541",
publisher = "Wiley-Blackwell",
number = "5",
}