Mapping and confirmation of two genes conferring resistance to soybean rust (Phakopsora pachyrhizi) in the soybean line UG-5 (Glycine max)

Chandra Paul; Glen L. Hartman; Brian W. Diers; David R. Walker

doi:10.1111/pbr.12854

Mapping and confirmation of two genes conferring resistance to soybean rust (Phakopsora pachyrhizi) in the soybean line UG-5 (Glycine max)

Chandra Paul, Glen L. Hartman, Brian W. Diers, David R. Walker

Research output: Contribution to journal › Article › peer-review

Abstract

Development of durable resistance to soybean rust (SBR) is challenging due to the pathogenic diversity of Phakopsora pachyrhizi populations. The objective of this research was to investigate and confirm the genomic locations of Rpp genes in the Ugandan line UG-5 that confer resistance to different SBR pathotypes. Bulked segregant analysis revealed two genomic regions associated with resistance in a cross with rust-susceptible 'Williams 82'. Composite interval mapping in the F₂ and F_2:3 populations had a LOD score of 48.7 in a region 0.38 cM away from the estimated location of the Rpp1 locus on chromosome (Chr.) 18. An approximately 23-Kbp interval spanning the Rpp1 locus was flanked by SNP markers ss715632313 and ss715632318. Another interval was identified at the Rpp3 locus on Chr. 6 between markers Satt100 and ss715594488 (2.4 cM) in the F₂ population and between Satt100 and ss715594874 (4.3 cM) in the F_2:3 population, with a maximum LOD score of 25.6. UG-5 was thus confirmed to have SBR resistance genes at the Rpp1 and Rpp3 loci that can be pyramided into other elite cultivars.

Original language	English (US)
Pages (from-to)	932-942
Number of pages	11
Journal	Plant Breeding
Volume	139
Issue number	5
DOIs	https://doi.org/10.1111/pbr.12854
State	Published - Oct 1 2020

Keywords

Phakopsora pachyrhizi
Rpp genes
bulked segregant analysis
rust resistance
soybean
soybean rust

ASJC Scopus subject areas

Agronomy and Crop Science
Genetics
Plant Science

Online availability

10.1111/pbr.12854

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title = "Mapping and confirmation of two genes conferring resistance to soybean rust (Phakopsora pachyrhizi) in the soybean line UG-5 (Glycine max)",

abstract = "Development of durable resistance to soybean rust (SBR) is challenging due to the pathogenic diversity of Phakopsora pachyrhizi populations. The objective of this research was to investigate and confirm the genomic locations of Rpp genes in the Ugandan line UG-5 that confer resistance to different SBR pathotypes. Bulked segregant analysis revealed two genomic regions associated with resistance in a cross with rust-susceptible 'Williams 82'. Composite interval mapping in the F2 and F2:3 populations had a LOD score of 48.7 in a region 0.38 cM away from the estimated location of the Rpp1 locus on chromosome (Chr.) 18. An approximately 23-Kbp interval spanning the Rpp1 locus was flanked by SNP markers ss715632313 and ss715632318. Another interval was identified at the Rpp3 locus on Chr. 6 between markers Satt100 and ss715594488 (2.4 cM) in the F2 population and between Satt100 and ss715594874 (4.3 cM) in the F2:3 population, with a maximum LOD score of 25.6. UG-5 was thus confirmed to have SBR resistance genes at the Rpp1 and Rpp3 loci that can be pyramided into other elite cultivars.",

keywords = "Phakopsora pachyrhizi, Rpp genes, bulked segregant analysis, rust resistance, soybean, soybean rust",

author = "Chandra Paul and Hartman, {Glen L.} and Diers, {Brian W.} and Walker, {David R.}",

note = "Publisher Copyright: Published 2020. This article is a U.S. Government work and is in the public domain in the USA.",

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AU - Paul, Chandra

AU - Hartman, Glen L.

AU - Diers, Brian W.

AU - Walker, David R.

N1 - Publisher Copyright: Published 2020. This article is a U.S. Government work and is in the public domain in the USA.

PY - 2020/10/1

Y1 - 2020/10/1

N2 - Development of durable resistance to soybean rust (SBR) is challenging due to the pathogenic diversity of Phakopsora pachyrhizi populations. The objective of this research was to investigate and confirm the genomic locations of Rpp genes in the Ugandan line UG-5 that confer resistance to different SBR pathotypes. Bulked segregant analysis revealed two genomic regions associated with resistance in a cross with rust-susceptible 'Williams 82'. Composite interval mapping in the F2 and F2:3 populations had a LOD score of 48.7 in a region 0.38 cM away from the estimated location of the Rpp1 locus on chromosome (Chr.) 18. An approximately 23-Kbp interval spanning the Rpp1 locus was flanked by SNP markers ss715632313 and ss715632318. Another interval was identified at the Rpp3 locus on Chr. 6 between markers Satt100 and ss715594488 (2.4 cM) in the F2 population and between Satt100 and ss715594874 (4.3 cM) in the F2:3 population, with a maximum LOD score of 25.6. UG-5 was thus confirmed to have SBR resistance genes at the Rpp1 and Rpp3 loci that can be pyramided into other elite cultivars.

AB - Development of durable resistance to soybean rust (SBR) is challenging due to the pathogenic diversity of Phakopsora pachyrhizi populations. The objective of this research was to investigate and confirm the genomic locations of Rpp genes in the Ugandan line UG-5 that confer resistance to different SBR pathotypes. Bulked segregant analysis revealed two genomic regions associated with resistance in a cross with rust-susceptible 'Williams 82'. Composite interval mapping in the F2 and F2:3 populations had a LOD score of 48.7 in a region 0.38 cM away from the estimated location of the Rpp1 locus on chromosome (Chr.) 18. An approximately 23-Kbp interval spanning the Rpp1 locus was flanked by SNP markers ss715632313 and ss715632318. Another interval was identified at the Rpp3 locus on Chr. 6 between markers Satt100 and ss715594488 (2.4 cM) in the F2 population and between Satt100 and ss715594874 (4.3 cM) in the F2:3 population, with a maximum LOD score of 25.6. UG-5 was thus confirmed to have SBR resistance genes at the Rpp1 and Rpp3 loci that can be pyramided into other elite cultivars.

KW - Phakopsora pachyrhizi

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Mapping and confirmation of two genes conferring resistance to soybean rust (Phakopsora pachyrhizi) in the soybean line UG-5 (Glycine max)

Abstract

Keywords

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