@inbook{cd1dd08e02d346ec9cd2e79f29e150f0,
title = "Manufacturing and delivering genome-editing proteins",
abstract = "Genome-editing technologies have revolutionized the biomedical sciences by providing researchers with the ability to quickly and efficiently modify genes. While programmable nucleases can be introduced into cells using a variety of techniques, their delivery as purified proteins is an effective approach for limiting off-target effects. Here, we describe step-by-step procedures for manufacturing and delivering genome-modifying proteins—including Cas9 ribonucleoproteins (RNPs) and TALE and zinc-finger nucleases—into mammalian cells. Protocols for combining Cas9 RNP with naturally recombinogenic adeno-associated virus (AAV) donor vectors for the seamless insertion of transgenes by homology-directed genome editing are also provided.",
keywords = "CRISPR, Genome editing, Protein delivery, RNP, TALENs, ZFNs",
author = "Jia Liu and Liang, {Ya jun} and Ren, {Pei ling} and Thomas Gaj",
note = "This work was supported by Natural Science Foundation of China (No. 31600686 to J.L.) and ShanghaiTech University (Startup fund to the Laboratory of ADC Chemistry).",
year = "2018",
doi = "10.1007/978-1-4939-8799-3_19",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "253--273",
booktitle = "Methods in Molecular Biology",
}