Male germ cell-specific expression of a novel Patched-domain containing gene Ptchd3

Jun Fan, Hiroto Akabane, Xuehai Zheng, Xuan Zhou, Li Zhang, Qiang Liu, Yong Lian Zhang, Jing Yang, Guo Zhang Zhu

Research output: Contribution to journalArticle

Abstract

The Hedgehog (Hh) signaling pathway plays an important role in various biological processes, including pattern formation, cell fate determination, proliferation, and differentiation. Hh function is mediated through its membrane receptor Patched. Herein, we have characterized a novel Patched-domain containing gene Ptchd3 in mouse. Messenger RNA of Ptchd3 was exclusively detected in the testis, and existed in two isoforms Ptchd3a and Ptchd3b. The expression of these two mRNA isoforms was shown to be developmentally regulated in testes, and specifically found in male germ cells. Further analysis revealed that the Ptchd3 protein was located on the midpiece of mouse, rat and human sperm. Collectively, these results indicate that Ptchd3 is a novel male germ cell-specific gene and may be involved in the Hh signaling to regulate sperm development and/or sperm function.

Original languageEnglish (US)
Pages (from-to)757-761
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume363
Issue number3
DOIs
StatePublished - Nov 23 2007
Externally publishedYes

Keywords

  • Hedgehog signaling
  • Male germ cell-specific
  • Midpiece
  • Patched
  • Ptchd3
  • Sperm

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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