Abstract

Mass spectrometry imaging (MSI) provides the ability to detect and identify a broad range of analytes and their spatial distributions from a variety of sample types, including tissue sections. Here we describe an approach for probing neuropeptides from sparse cell cultures using matrixassisted laser desorption/ionization time-of-flight (MALDI-TOF) MSI-at single cell spatial resolution-in both MS and tandem MS modes. Cultures of Aplysia californica neurons are grown on an array of glass beads embedded in a stretchable layer of Parafilm M. As the membrane is stretched, the beads/neurons are separated physically and the separated beads/ neurons analyzed via MALDI TOF MS. Compared with direct MS imaging of samples, the stretching procedure enhances analyte extraction and incorporation into the MALDI matrix, with negligible analyte spread between separated beads. MALDI tandem MSI using the stretched imaging approach yields localization maps of both parent and fragment ions from Aplysia pedal peptide, thereby confirming peptide identification. This methodology represents a flexible platform for MSI investigation of a variety of cell cultures, including functioning neuronal networks.

Original languageEnglish (US)
Pages (from-to)828-836
Number of pages9
JournalJournal of the American Society for Mass Spectrometry
Volume22
Issue number5
DOIs
StatePublished - May 1 2011

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Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Cell culture
Mass spectrometry
Aplysia
Mass Spectrometry
Cell Culture Techniques
Imaging techniques
Neurons
Tandem Mass Spectrometry
Neuropeptides
Paraffin
Glass
Lasers
Ions
Peptides
Membranes
Spatial distribution
Stretching
Ionization
Desorption

Keywords

  • Aplysia
  • Cell cultures
  • MALDI
  • MSI

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

Cite this

MALDI mass spectrometry imaging of neuronal cell cultures. / Zimmerman, Tyler A.; Rubakhin, Stanislav S.; Sweedler, Jonathan V.

In: Journal of the American Society for Mass Spectrometry, Vol. 22, No. 5, 01.05.2011, p. 828-836.

Research output: Contribution to journalArticle

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