MALDI-guided SIMS: Multiscale imaging of metabolites in bacterial biofilms

Eric J. Lanni, Rachel N. Masyuko, Callan M. Driscoll, Jordan T. Aerts, Joshua D. Shrout, Paul W. Bohn, Jonathan V. Sweedler

Research output: Contribution to journalArticle

Abstract

Mass spectrometry imaging (MSI) is a versatile tool for visualizing molecular distributions in complex biological specimens, but locating microscopic chemical features of interest can be challenging in samples that lack a well-defined anatomy. To address this issue, we developed a correlated imaging approach that begins with performing matrix-assisted laser desorption/ionization (MALDI) MSI to obtain low-resolution molecular maps of a sample. The resulting maps are then used to direct subsequent microscopic secondary ion mass spectrometry (SIMS) imaging and tandem mass spectrometry (MS/MS) experiments to examine selected chemical regions of interest. By employing MALDI undersampling, the sample surface is left mostly unperturbed and available for the SIMS analysis, while also generating an ablation array that can be used for navigation in SIMS. We validated this MALDI-guided SIMS approach using cultured biofilms of the opportunistic pathogen Pseudomonas aeruginosa; bioactive secondary metabolites, including rhamnolipids and quinolones, were detected and visualized on both macro- and microscopic size scales. MSI mass assignments were confirmed with in situ MALDI MS/MS and capillary electrophoresis?electrospray ionization MS/MS analysis of biofilm extracts. Two strains of P. aeruginosa were compared, wild type and a quorum sensing mutant, and differences in metabolite abundance and distribution were observed.

Original languageEnglish (US)
Pages (from-to)9139-9145
Number of pages7
JournalAnalytical chemistry
Volume86
Issue number18
DOIs
StatePublished - Sep 1 2014

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Biofilms
Secondary ion mass spectrometry
Metabolites
Ionization
Desorption
Mass spectrometry
Imaging techniques
Lasers
Capillary electrophoresis
Electrospray ionization
Quinolones
Pathogens
Ablation
Macros
Navigation
Experiments

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Lanni, E. J., Masyuko, R. N., Driscoll, C. M., Aerts, J. T., Shrout, J. D., Bohn, P. W., & Sweedler, J. V. (2014). MALDI-guided SIMS: Multiscale imaging of metabolites in bacterial biofilms. Analytical chemistry, 86(18), 9139-9145. https://doi.org/10.1021/ac5020222

MALDI-guided SIMS : Multiscale imaging of metabolites in bacterial biofilms. / Lanni, Eric J.; Masyuko, Rachel N.; Driscoll, Callan M.; Aerts, Jordan T.; Shrout, Joshua D.; Bohn, Paul W.; Sweedler, Jonathan V.

In: Analytical chemistry, Vol. 86, No. 18, 01.09.2014, p. 9139-9145.

Research output: Contribution to journalArticle

Lanni, EJ, Masyuko, RN, Driscoll, CM, Aerts, JT, Shrout, JD, Bohn, PW & Sweedler, JV 2014, 'MALDI-guided SIMS: Multiscale imaging of metabolites in bacterial biofilms', Analytical chemistry, vol. 86, no. 18, pp. 9139-9145. https://doi.org/10.1021/ac5020222
Lanni EJ, Masyuko RN, Driscoll CM, Aerts JT, Shrout JD, Bohn PW et al. MALDI-guided SIMS: Multiscale imaging of metabolites in bacterial biofilms. Analytical chemistry. 2014 Sep 1;86(18):9139-9145. https://doi.org/10.1021/ac5020222
Lanni, Eric J. ; Masyuko, Rachel N. ; Driscoll, Callan M. ; Aerts, Jordan T. ; Shrout, Joshua D. ; Bohn, Paul W. ; Sweedler, Jonathan V. / MALDI-guided SIMS : Multiscale imaging of metabolites in bacterial biofilms. In: Analytical chemistry. 2014 ; Vol. 86, No. 18. pp. 9139-9145.
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