Malat1 is not an essential component of nuclear speckles in mice

Shinichi Nakagawa, Joanna Y. Ip, Go Shioi, Vidisha Tripathi, Xinying Zong, Tetsuro Hirose, Kannanganattu V. Prasanth

Research output: Contribution to journalArticle

Abstract

Malat1 is an abundant long, noncoding RNA that localizes to nuclear bodies known as nuclear speckles, which contain a distinct set of pre-mRNA processing factors. Previous studies in cell culture have demonstrated that Malat1 interacts with pre-mRNA splicing factors, including the serine- and arginine-rich (SR) family of proteins, and regulates a variety of biological processes, including cancer cell migration, synapse formation, cell cycle progression, and responses to serum stimulation. To address the physiological function of Malat1 in a living organism, we generated Malat1-knockout (KO) mice using homologous recombination. Unexpectedly, the Malat1-KO mice were viable and fertile, showing no apparent phenotypes. Nuclear speckle markers were also correctly localized in cells that lacked Malat1. However, the cellular levels of another long, noncoding RNA - Neat1 - which is an architectural component of nuclear bodies known as paraspeckles, were down-regulated in a particular set of tissues and cells lacking Malat1. We propose that Malat1 is not essential in living mice maintained under normal laboratory conditions and that its function becomes apparent only in specific cell types and under particular conditions. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)1487-1499
Number of pages13
JournalRNA
Volume18
Issue number8
DOIs
StatePublished - Aug 1 2012

Fingerprint

Long Noncoding RNA
RNA Precursors
Knockout Mice
Biological Phenomena
Homologous Recombination
Synapses
Cell Movement
Cell Cycle
Cell Culture Techniques
Phenotype
Serum
Neoplasms
Proteins
Serine-Arginine Splicing Factors

Keywords

  • Malat1
  • Nuclear speckles
  • Paraspeckles

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Nakagawa, S., Ip, J. Y., Shioi, G., Tripathi, V., Zong, X., Hirose, T., & Prasanth, K. V. (2012). Malat1 is not an essential component of nuclear speckles in mice. RNA, 18(8), 1487-1499. https://doi.org/10.1261/rna.033217.112

Malat1 is not an essential component of nuclear speckles in mice. / Nakagawa, Shinichi; Ip, Joanna Y.; Shioi, Go; Tripathi, Vidisha; Zong, Xinying; Hirose, Tetsuro; Prasanth, Kannanganattu V.

In: RNA, Vol. 18, No. 8, 01.08.2012, p. 1487-1499.

Research output: Contribution to journalArticle

Nakagawa, S, Ip, JY, Shioi, G, Tripathi, V, Zong, X, Hirose, T & Prasanth, KV 2012, 'Malat1 is not an essential component of nuclear speckles in mice', RNA, vol. 18, no. 8, pp. 1487-1499. https://doi.org/10.1261/rna.033217.112
Nakagawa S, Ip JY, Shioi G, Tripathi V, Zong X, Hirose T et al. Malat1 is not an essential component of nuclear speckles in mice. RNA. 2012 Aug 1;18(8):1487-1499. https://doi.org/10.1261/rna.033217.112
Nakagawa, Shinichi ; Ip, Joanna Y. ; Shioi, Go ; Tripathi, Vidisha ; Zong, Xinying ; Hirose, Tetsuro ; Prasanth, Kannanganattu V. / Malat1 is not an essential component of nuclear speckles in mice. In: RNA. 2012 ; Vol. 18, No. 8. pp. 1487-1499.
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