Abstract

Magnetomotive microscopy techniques are introduced to investigate cell dynamics and biomechanics. These techniques are based on magnetomotive transducers present in cells and optical coherence imaging techniques. In this study, magnetomotive transducers include magnetic nanoparticles (MNPs) and fluorescently labeled magnetic microspheres, while the optical coherence imaging techniques include integrated optical coherence (OCM)and multiphoton (MPM) microscopy,and diffraction phase microscopy (DPM). Samples used in this study are murine macrophage cells in culture that were incubated with magnetomotive transducers. MPMis used to visualize multifunctional microspheres based on their fluorescence, while magnetomotive OCM detects sinusoidal displacements of the sample induced by a magnetic field. DPM is used to image single cells at a lower frequency magnetic excitation, and with its Fourier transform light scattering (FTLS) analysis, oscillation amplitude is obtained, indicating the relative biomechanical properties of macrophage cells. These magnetomotive microscopy method shave potential to be used to image and measure cell dynamics and biomechanical properties. The ability to measure and understand biomechanical properties of cells and their microenvironments, especially for tumor cells, is of great importance and may provide insight for diagnostic and subsequently therapeutic interventions.

Original languageEnglish (US)
Title of host publicationOptical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV
DOIs
StatePublished - Apr 12 2011
EventOptical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV - San Francisco, CA, United States
Duration: Jan 24 2011Jan 26 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7889
ISSN (Print)1605-7422

Other

OtherOptical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV
CountryUnited States
CitySan Francisco, CA
Period1/24/111/26/11

Keywords

  • cell biomechanics
  • diffraction phase microscopy
  • Magnetic tweezers
  • optical coherence microscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Liang, X., Graf, B. W., John, R., Crecea, V., Nguyen, F. T., Ding, H., Song, H. M., Popescu, G., Wei, A., & Boppart, S. A. (2011). Magnetomotive optical coherence microscopy for cell dynamics and biomechanics. In Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV [788926] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7889). https://doi.org/10.1117/12.875139