Magnetomotive optical coherence microscopy for cell dynamics and biomechanics

Xing Liang, Benedikt W. Graf, Renu John, Vasilica Crecea, Freddy T. Nguyen, Huafeng Ding, Hyon Min Song, Gabriel Popescu, Alexander Wei, Stephen A. Boppart

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Magnetomotive microscopy techniques are introduced to investigate cell dynamics and biomechanics. These techniques are based on magnetomotive transducers present in cells and optical coherence imaging techniques. In this study, magnetomotive transducers include magnetic nanoparticles (MNPs) and fluorescently labeled magnetic microspheres, while the optical coherence imaging techniques include integrated optical coherence (OCM)and multiphoton (MPM) microscopy,and diffraction phase microscopy (DPM). Samples used in this study are murine macrophage cells in culture that were incubated with magnetomotive transducers. MPMis used to visualize multifunctional microspheres based on their fluorescence, while magnetomotive OCM detects sinusoidal displacements of the sample induced by a magnetic field. DPM is used to image single cells at a lower frequency magnetic excitation, and with its Fourier transform light scattering (FTLS) analysis, oscillation amplitude is obtained, indicating the relative biomechanical properties of macrophage cells. These magnetomotive microscopy method shave potential to be used to image and measure cell dynamics and biomechanical properties. The ability to measure and understand biomechanical properties of cells and their microenvironments, especially for tumor cells, is of great importance and may provide insight for diagnostic and subsequently therapeutic interventions.

Original languageEnglish (US)
Title of host publicationOptical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV
Volume7889
DOIs
StatePublished - Apr 12 2011
EventOptical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV - San Francisco, CA, United States
Duration: Jan 24 2011Jan 26 2011

Other

OtherOptical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV
CountryUnited States
CitySan Francisco, CA
Period1/24/111/26/11

Fingerprint

biodynamics
Biomechanics
Biomechanical Phenomena
Microscopy
Microscopic examination
microscopy
Transducers
cells
Macrophages
Microspheres
Optical Imaging
macrophages
Diffraction
imaging techniques
Imaging techniques
magnetic transducers
transducers
Cellular Microenvironment
Cell culture
Light scattering

Keywords

  • cell biomechanics
  • diffraction phase microscopy
  • Magnetic tweezers
  • optical coherence microscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Liang, X., Graf, B. W., John, R., Crecea, V., Nguyen, F. T., Ding, H., ... Boppart, S. A. (2011). Magnetomotive optical coherence microscopy for cell dynamics and biomechanics. In Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV (Vol. 7889). [788926] https://doi.org/10.1117/12.875139

Magnetomotive optical coherence microscopy for cell dynamics and biomechanics. / Liang, Xing; Graf, Benedikt W.; John, Renu; Crecea, Vasilica; Nguyen, Freddy T.; Ding, Huafeng; Song, Hyon Min; Popescu, Gabriel; Wei, Alexander; Boppart, Stephen A.

Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV. Vol. 7889 2011. 788926.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Liang, X, Graf, BW, John, R, Crecea, V, Nguyen, FT, Ding, H, Song, HM, Popescu, G, Wei, A & Boppart, SA 2011, Magnetomotive optical coherence microscopy for cell dynamics and biomechanics. in Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV. vol. 7889, 788926, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV, San Francisco, CA, United States, 1/24/11. https://doi.org/10.1117/12.875139
Liang X, Graf BW, John R, Crecea V, Nguyen FT, Ding H et al. Magnetomotive optical coherence microscopy for cell dynamics and biomechanics. In Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV. Vol. 7889. 2011. 788926 https://doi.org/10.1117/12.875139
Liang, Xing ; Graf, Benedikt W. ; John, Renu ; Crecea, Vasilica ; Nguyen, Freddy T. ; Ding, Huafeng ; Song, Hyon Min ; Popescu, Gabriel ; Wei, Alexander ; Boppart, Stephen A. / Magnetomotive optical coherence microscopy for cell dynamics and biomechanics. Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV. Vol. 7889 2011.
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