TY - GEN
T1 - Magnetomotive contrast in optical coherence tomography for detecting early-stage atherosclerosis using targeted microspheres
AU - Ahmad, Adeel
AU - Kim, Jong S.
AU - Li, Joanne
AU - Rasio, Jonathan
AU - Hubler, Zita
AU - Chaney, Eric J.
AU - Marjanovic, Marina
AU - Suslick, Kenneth S
AU - Boppart, Stephen A.
PY - 2012
Y1 - 2012
N2 - In this work, we show that molecularly-sensitive contrast can be generated in OCT using targeted multifunctional magnetic microspheres. These microspheres were engineered to target the ΑvΒ3 integrin for the localization of atherosclerotic lesions in excised aortas from a rabbit animal model. The aortas were extracted and placed in a flow chamber, which mimicked the physiological blood flow conditions in the living rabbit. Magnetic microspheres were perfused through the aorta inside the flow chamber and catheter-based OCT imaging, magnetomotive optical coherence tomography (MM-OCT), fluorescence confocal, and bright field microscopy was performed on the ex vivo aorta specimens for localizing the microspheres. Results showed successful targeting of the functionalized microspheres to early atherosclerotic lesions, and good co-registration of MM-OCT signal with confocal microscopy.
AB - In this work, we show that molecularly-sensitive contrast can be generated in OCT using targeted multifunctional magnetic microspheres. These microspheres were engineered to target the ΑvΒ3 integrin for the localization of atherosclerotic lesions in excised aortas from a rabbit animal model. The aortas were extracted and placed in a flow chamber, which mimicked the physiological blood flow conditions in the living rabbit. Magnetic microspheres were perfused through the aorta inside the flow chamber and catheter-based OCT imaging, magnetomotive optical coherence tomography (MM-OCT), fluorescence confocal, and bright field microscopy was performed on the ex vivo aorta specimens for localizing the microspheres. Results showed successful targeting of the functionalized microspheres to early atherosclerotic lesions, and good co-registration of MM-OCT signal with confocal microscopy.
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U2 - 10.1364/biomed.2012.btu3a.85
DO - 10.1364/biomed.2012.btu3a.85
M3 - Conference contribution
AN - SCOPUS:84890573385
SN - 9781557529428
T3 - Biomedical Optics, BIOMED 2012
SP - BTu3A.85
BT - Biomedical Optics, BIOMED 2012
PB - Optical Society of America (OSA)
T2 - Biomedical Optics, BIOMED 2012
Y2 - 28 April 2012 through 2 May 2012
ER -