Magnetic protein microspheres as dynamic contrast agents for magnetomotive optical coherence tomography

Freddy T. Nguyen, Elizabeth M. Dibbern, Eric J. Chaney, Amy L. Oldenburg, Kenneth S Suslick, Stephen A. Boppart

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Optical coherence tomography (OCT) is an emerging biomedical imaging modality that has been developed over the last 15 years. More recently, OCT has been used for the intraoperative imaging of tumor margins in breast cancer and axillary lymph nodes providing a real time in-vivo assessment of the tissue morphology. Traditional OCT images are limited by only being able to observe morphological structures. As diagnostic medicine continues to push for earlier detection, one must develop functional imaging modalities that would detect molecular information in-vivo allowing a real-time microscopic analysis of the tissue specimen. A novel modality of OCT called magnetomotive-OCT (MMOCT) has been developed by our group, employing an induced modulated magnetic field with a magnetic contrast agent to create the added contrast to structural OCT images. Modified protein microspheres with a BSA protein shell functionalized with RGD peptide sequences for targeting and an oil core have been designed and synthesized. Magnetic nanoparticles (Fe3O4) and Nile Red dye have been encapsulated into its oil core. These microspheres have previously been demonstrated to target cancer cells by functionalizing them with a layer of RGD peptides and could be functionalized with monoclonal antibodies. Preliminary results show that these magnetic microspheres, which are 2.0-5.0 microns in size, are readily detectable under MM-OCT when embedded in a 5% agarose gel, in a 3-D scaffold of macrophage cells previously incubated with the microspheres, and when injected in-vivo into a tumor from an NMU-carcinogen rat animal model for breast cancer.

Original languageEnglish (US)
Title of host publicationMolecular Probes for Biomedical Applications II
DOIs
StatePublished - Apr 21 2008
EventMolecular Probes for Biomedical Applications II - San Jose, CA, United States
Duration: Jan 21 2008Jan 22 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6867
ISSN (Print)1605-7422

Other

OtherMolecular Probes for Biomedical Applications II
CountryUnited States
CitySan Jose, CA
Period1/21/081/22/08

Fingerprint

Optical tomography
Microspheres
Proteins
Imaging techniques
Peptides
Tumors
Tissue
Carcinogens
Monoclonal antibodies
Macrophages
Scaffolds (biology)
Medicine
Rats
Animals
Gels
Dyes
Cells
Magnetic fields
Nanoparticles

Keywords

  • Atherosclerosis
  • Cancer
  • Contrast agent
  • Microspheres
  • Optical coherence tomography

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Nguyen, F. T., Dibbern, E. M., Chaney, E. J., Oldenburg, A. L., Suslick, K. S., & Boppart, S. A. (2008). Magnetic protein microspheres as dynamic contrast agents for magnetomotive optical coherence tomography. In Molecular Probes for Biomedical Applications II [68670F] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6867). https://doi.org/10.1117/12.762428

Magnetic protein microspheres as dynamic contrast agents for magnetomotive optical coherence tomography. / Nguyen, Freddy T.; Dibbern, Elizabeth M.; Chaney, Eric J.; Oldenburg, Amy L.; Suslick, Kenneth S; Boppart, Stephen A.

Molecular Probes for Biomedical Applications II. 2008. 68670F (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6867).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nguyen, FT, Dibbern, EM, Chaney, EJ, Oldenburg, AL, Suslick, KS & Boppart, SA 2008, Magnetic protein microspheres as dynamic contrast agents for magnetomotive optical coherence tomography. in Molecular Probes for Biomedical Applications II., 68670F, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6867, Molecular Probes for Biomedical Applications II, San Jose, CA, United States, 1/21/08. https://doi.org/10.1117/12.762428
Nguyen FT, Dibbern EM, Chaney EJ, Oldenburg AL, Suslick KS, Boppart SA. Magnetic protein microspheres as dynamic contrast agents for magnetomotive optical coherence tomography. In Molecular Probes for Biomedical Applications II. 2008. 68670F. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.762428
Nguyen, Freddy T. ; Dibbern, Elizabeth M. ; Chaney, Eric J. ; Oldenburg, Amy L. ; Suslick, Kenneth S ; Boppart, Stephen A. / Magnetic protein microspheres as dynamic contrast agents for magnetomotive optical coherence tomography. Molecular Probes for Biomedical Applications II. 2008. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
@inproceedings{c418844b76e44e5e861ef858d32e9984,
title = "Magnetic protein microspheres as dynamic contrast agents for magnetomotive optical coherence tomography",
abstract = "Optical coherence tomography (OCT) is an emerging biomedical imaging modality that has been developed over the last 15 years. More recently, OCT has been used for the intraoperative imaging of tumor margins in breast cancer and axillary lymph nodes providing a real time in-vivo assessment of the tissue morphology. Traditional OCT images are limited by only being able to observe morphological structures. As diagnostic medicine continues to push for earlier detection, one must develop functional imaging modalities that would detect molecular information in-vivo allowing a real-time microscopic analysis of the tissue specimen. A novel modality of OCT called magnetomotive-OCT (MMOCT) has been developed by our group, employing an induced modulated magnetic field with a magnetic contrast agent to create the added contrast to structural OCT images. Modified protein microspheres with a BSA protein shell functionalized with RGD peptide sequences for targeting and an oil core have been designed and synthesized. Magnetic nanoparticles (Fe3O4) and Nile Red dye have been encapsulated into its oil core. These microspheres have previously been demonstrated to target cancer cells by functionalizing them with a layer of RGD peptides and could be functionalized with monoclonal antibodies. Preliminary results show that these magnetic microspheres, which are 2.0-5.0 microns in size, are readily detectable under MM-OCT when embedded in a 5{\%} agarose gel, in a 3-D scaffold of macrophage cells previously incubated with the microspheres, and when injected in-vivo into a tumor from an NMU-carcinogen rat animal model for breast cancer.",
keywords = "Atherosclerosis, Cancer, Contrast agent, Microspheres, Optical coherence tomography",
author = "Nguyen, {Freddy T.} and Dibbern, {Elizabeth M.} and Chaney, {Eric J.} and Oldenburg, {Amy L.} and Suslick, {Kenneth S} and Boppart, {Stephen A.}",
year = "2008",
month = "4",
day = "21",
doi = "10.1117/12.762428",
language = "English (US)",
isbn = "9780819470423",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Molecular Probes for Biomedical Applications II",

}

TY - GEN

T1 - Magnetic protein microspheres as dynamic contrast agents for magnetomotive optical coherence tomography

AU - Nguyen, Freddy T.

AU - Dibbern, Elizabeth M.

AU - Chaney, Eric J.

AU - Oldenburg, Amy L.

AU - Suslick, Kenneth S

AU - Boppart, Stephen A.

PY - 2008/4/21

Y1 - 2008/4/21

N2 - Optical coherence tomography (OCT) is an emerging biomedical imaging modality that has been developed over the last 15 years. More recently, OCT has been used for the intraoperative imaging of tumor margins in breast cancer and axillary lymph nodes providing a real time in-vivo assessment of the tissue morphology. Traditional OCT images are limited by only being able to observe morphological structures. As diagnostic medicine continues to push for earlier detection, one must develop functional imaging modalities that would detect molecular information in-vivo allowing a real-time microscopic analysis of the tissue specimen. A novel modality of OCT called magnetomotive-OCT (MMOCT) has been developed by our group, employing an induced modulated magnetic field with a magnetic contrast agent to create the added contrast to structural OCT images. Modified protein microspheres with a BSA protein shell functionalized with RGD peptide sequences for targeting and an oil core have been designed and synthesized. Magnetic nanoparticles (Fe3O4) and Nile Red dye have been encapsulated into its oil core. These microspheres have previously been demonstrated to target cancer cells by functionalizing them with a layer of RGD peptides and could be functionalized with monoclonal antibodies. Preliminary results show that these magnetic microspheres, which are 2.0-5.0 microns in size, are readily detectable under MM-OCT when embedded in a 5% agarose gel, in a 3-D scaffold of macrophage cells previously incubated with the microspheres, and when injected in-vivo into a tumor from an NMU-carcinogen rat animal model for breast cancer.

AB - Optical coherence tomography (OCT) is an emerging biomedical imaging modality that has been developed over the last 15 years. More recently, OCT has been used for the intraoperative imaging of tumor margins in breast cancer and axillary lymph nodes providing a real time in-vivo assessment of the tissue morphology. Traditional OCT images are limited by only being able to observe morphological structures. As diagnostic medicine continues to push for earlier detection, one must develop functional imaging modalities that would detect molecular information in-vivo allowing a real-time microscopic analysis of the tissue specimen. A novel modality of OCT called magnetomotive-OCT (MMOCT) has been developed by our group, employing an induced modulated magnetic field with a magnetic contrast agent to create the added contrast to structural OCT images. Modified protein microspheres with a BSA protein shell functionalized with RGD peptide sequences for targeting and an oil core have been designed and synthesized. Magnetic nanoparticles (Fe3O4) and Nile Red dye have been encapsulated into its oil core. These microspheres have previously been demonstrated to target cancer cells by functionalizing them with a layer of RGD peptides and could be functionalized with monoclonal antibodies. Preliminary results show that these magnetic microspheres, which are 2.0-5.0 microns in size, are readily detectable under MM-OCT when embedded in a 5% agarose gel, in a 3-D scaffold of macrophage cells previously incubated with the microspheres, and when injected in-vivo into a tumor from an NMU-carcinogen rat animal model for breast cancer.

KW - Atherosclerosis

KW - Cancer

KW - Contrast agent

KW - Microspheres

KW - Optical coherence tomography

UR - http://www.scopus.com/inward/record.url?scp=42149164295&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42149164295&partnerID=8YFLogxK

U2 - 10.1117/12.762428

DO - 10.1117/12.762428

M3 - Conference contribution

AN - SCOPUS:42149164295

SN - 9780819470423

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Molecular Probes for Biomedical Applications II

ER -