Magnetic Focus Lateral Flow Sensor for Detection of Cervical Cancer Biomarkers

Wen Ren, Sulma I. Mohammed, Steven Wereley, Joseph Maria Kumar Irudayaraj

Research output: Contribution to journalArticle

Abstract

We report on a magnetic focus lateral flow biosensor (mLFS) for ultrasensitive detection of protein biomarkers in a practical format. With valosin-containing protein as a target protein, we show that the developed mLFS concept could detect as low as 25 fg/mL with magnetic focus to enhance target capture efficiency to deliver a 10 6 -fold improvement in sensitivity compared to that of conventional lateral flow (LF) systems. The conceptualized strategy utilizes a simple magnet placed beneath the three-dimensional printed LF device to concentrate the targets at the signal zone without any additional instrumentation. In addition, protein mixtures extracted from the tissue of cervical cancer patients was also utilized to validate the sensor. To investigate the effect of magnetic focus on sensitivity, surface-enhanced Raman spectroscopy and dark-field imaging was utilized to characterize the distribution and movement of Fe 3 O 4 core-Au shell nanoprobes in a model LF strip. Our experiments show that the magnetic focus results in an increased interaction time between the magnetic probe-labeled targets and the capture antibody, yielding a higher capture efficiency, allowing for ultrasensitive detection of the target not possible before with LF. The proposed mLFS can be utilized to detect a range of trace protein biomarkers for early diagnosis and can be combined with diverse pretreatment and signal amplification steps to query complex samples.

Original languageEnglish (US)
Pages (from-to)2876-2884
Number of pages9
JournalAnalytical chemistry
Volume91
Issue number4
DOIs
StatePublished - Feb 19 2019

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Tumor Biomarkers
Sensors
Biomarkers
Proteins
Nanoprobes
Biosensors
Magnets
Amplification
Raman spectroscopy
Tissue
Imaging techniques
Antibodies
Experiments

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Magnetic Focus Lateral Flow Sensor for Detection of Cervical Cancer Biomarkers. / Ren, Wen; Mohammed, Sulma I.; Wereley, Steven; Irudayaraj, Joseph Maria Kumar.

In: Analytical chemistry, Vol. 91, No. 4, 19.02.2019, p. 2876-2884.

Research output: Contribution to journalArticle

Ren, Wen ; Mohammed, Sulma I. ; Wereley, Steven ; Irudayaraj, Joseph Maria Kumar. / Magnetic Focus Lateral Flow Sensor for Detection of Cervical Cancer Biomarkers. In: Analytical chemistry. 2019 ; Vol. 91, No. 4. pp. 2876-2884.
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