Magic-angle spinning recoupling techniques for distance determinations among spin-1/2 nuclei in solid peptides and proteins

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Magic-angle spinning (MAS) recoupling techniques for determining long-range (>4 Å) distances among spin-1/2 nuclei are reviewed. These MAS experiments were originally designed to examine individual dipolar interactions between spin pairs to yield a few high-precision distances between site-specifically labeled nuclei. In recent years, many new pulse sequences and analysis methods have been devised for the purpose of examining spin clusters of several spins and even uniformly 13C, 15N-labeled samples with dozens to hundreds of resolved resonances. Such experiments sometimes require a tradeoff of measurement precision (data quality) to examine a larger number of distances per sample (data quantity). Here we consider both heteronuclear and homonuclear sequences, and discuss - from a qualitative and pragmatic perspective - the requirements and relative merits of various techniques in terms of available samples and instrumentation. Approaches to measure many semiquantitative distances in multidimensional spectra are emphasized, as these techniques will likely play a prominent role in total structure determination of solid proteins by magic-angle spinning nuclear magnetic resonance methods.

Original languageEnglish (US)
Title of host publicationNMR Spectroscopy of Biological Solids
PublisherCRC Press
Number of pages38
ISBN (Electronic)9781420027617
ISBN (Print)9781574444964
StatePublished - Jan 1 2005



  • Distances
  • Magic-angle spinning
  • Peptides
  • Proteins
  • Recoupling
  • Solid-state NMR
  • Structure determination

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)
  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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