Macromolecule synthesis leading to cell division in Tetrahymena pyriformis after replacement of required amino acids

T. S. Burns, D. E. Buetow

Research output: Contribution to journalArticle

Abstract

Tetrahymena pyriformis W were brought to a nonmultiplying state by removal of required amino acids from their growth medium. After amino-acid replacement, the incorporation rates of H3-uridine, H3-thymidine and H3-leucine were measured by the autoradiographic method. Following amino-acid replacement, the first response was detected in RNA synthesis, then protein synthesis, then DNA synthesis and, lastly, in cell division. Amino-acid deprived cells showed a 23% net increase in DNA content, a result supporting the view of others that protein synthesis is not necessary for the initiation of DNA synthesis but is necessary for the maintainance of DNA synthesis.

Original languageEnglish (US)
Pages (from-to)87-96
Number of pages10
JournalArchiv für Mikrobiologie
Volume80
Issue number1
DOIs
StatePublished - Mar 1 1971

ASJC Scopus subject areas

  • Medicine(all)

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