Lysosome transport as a function of lysosome diameter

Debjyoti Bandyopadhyay, Austin Cyphersmith, Jairo A. Zapata, Y. Joseph Kim, Christine K. Payne

Research output: Contribution to journalArticlepeer-review

Abstract

Lysosomes are membrane-bound organelles responsible for the transport and degradation of intracellular and extracellular cargo. The intracellular motion of lysosomes is both diffusive and active, mediated by motor proteins moving lysosomes along microtubules. We sought to determine how lysosome diameter influences lysosome transport. We used osmotic swelling to double the diameter of lysosomes, creating a population of enlarged lysosomes. This allowed us to directly examine the intracellular transport of the same organelle as a function of diameter. Lysosome transport was measured using live cell fluorescence microscopy and single particle tracking. We find, as expected, the diffusive component of intracellular transport is decreased proportional to the increased lysosome diameter. Active transport of the enlarged lysosomes is not affected by the increased lysosome diameter.

Original languageEnglish (US)
Article numbere86847
JournalPloS one
Volume9
Issue number1
DOIs
StatePublished - Jan 31 2014
Externally publishedYes

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Agricultural and Biological Sciences
  • General

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