TY - JOUR
T1 - Low density contrast agents for x-ray phase contrast imaging
T2 - The use of ambient air for x-ray angiography of excised murine liver tissue
AU - Laperle, Christopher M.
AU - Hamilton, Theron J.
AU - Wintermeyer, Philip
AU - Walker, Evan J.
AU - Shi, Daxin
AU - Anastasio, Mark A.
AU - Derdak, Zoltan
AU - Wands, Jack R.
AU - Diebold, Gerald
AU - Rose-Petruck, Christoph
PY - 2008/12/7
Y1 - 2008/12/7
N2 - We report a new preparative method for providing contrast through reduction in electron density that is uniquely suited for propagation-based differential x-ray phase contrast imaging. The method, which results in an air or fluid filled vasculature, makes possible visualization of the smallest microvessels, roughly down to 15 νm, in an excised murine liver, while preserving the tissue for subsequent histological workup. We show the utility of spatial frequency filtering for increasing the visibility of minute features characteristic of phase contrast imaging, and the capability of tomographic reconstruction to reveal microvessel structure and three-dimensional visualization of the sample. The effect of water evaporation from livers during x-ray imaging on the visibility of blood vessels is delineated. The deformed vascular tree in a cancerous murine liver is imaged.
AB - We report a new preparative method for providing contrast through reduction in electron density that is uniquely suited for propagation-based differential x-ray phase contrast imaging. The method, which results in an air or fluid filled vasculature, makes possible visualization of the smallest microvessels, roughly down to 15 νm, in an excised murine liver, while preserving the tissue for subsequent histological workup. We show the utility of spatial frequency filtering for increasing the visibility of minute features characteristic of phase contrast imaging, and the capability of tomographic reconstruction to reveal microvessel structure and three-dimensional visualization of the sample. The effect of water evaporation from livers during x-ray imaging on the visibility of blood vessels is delineated. The deformed vascular tree in a cancerous murine liver is imaged.
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U2 - 10.1088/0031-9155/53/23/017
DO - 10.1088/0031-9155/53/23/017
M3 - Article
C2 - 19001700
AN - SCOPUS:58149216496
SN - 0031-9155
VL - 53
SP - 6911
EP - 6923
JO - Physics in medicine and biology
JF - Physics in medicine and biology
IS - 23
ER -