Low-cost, high-sensitivity SERS nano-bio-chip for kinase profiling, drug monitoring and environmental detection: A translational platform technology

Yi Chen, Logan Liu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The interaction of biomolecules and solid-state nanomaterials at the nano-bio interfaces is a long-lasting research topic in nanotechnology. Historically, fundamental problems, such as the electron transfer, energy transfer, and plasmonic interaction at the bio-nano interfaces, have been intensively studied, and revolutionary technologies, such as molecular electronics, peptide chips, nanoplasmonic sensors, have been created. With the combined effort of molecular dynamics simulation and surface-enhanced Raman spectroscopy, we studied the external electric field-induced conformation changes of dodecapeptide probes tethered to a nanostructured metallic surface. Through this study, we demonstrated a reversible manipulation of the biomolecule conformations as well as an in situ eletro-optical detection of the subnanometer conformational changes at the bio-nano interfaces. Based on the proof-of-concept established in this study, we further propose a novel nanophotonic peptide phosphorylation sensor for high-sensitive peptide kinase profiling. We have also demonstrated the same SERS nano-bio-chip can be used for environmental monitoring applications, such as detection of contaminants in drinking water at ultralow concentrates. The fabrication of this nanosensor is based on a single step, lithography-less nanomanufacturing process, which can produce hundreds of these chips in several minutes with nearly 100% yield and uniformity. Therefore, the demonstrated research can be readily translated into industrial mass productions.

Original languageEnglish (US)
Title of host publicationPlasmonics in Biology and Medicine XI
PublisherSPIE
ISBN (Print)9780819498700
DOIs
StatePublished - Jan 1 2014
EventPlasmonics in Biology and Medicine XI - San Francisco, CA, United States
Duration: Feb 1 2014Feb 2 2014

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8957
ISSN (Print)1605-7422

Other

OtherPlasmonics in Biology and Medicine XI
CountryUnited States
CitySan Francisco, CA
Period2/1/142/2/14

Keywords

  • electrically induced conformational change
  • molecular dynamics
  • Nano-bio interface
  • surface-enhanced Raman spectroscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Chen, Y., & Liu, L. (2014). Low-cost, high-sensitivity SERS nano-bio-chip for kinase profiling, drug monitoring and environmental detection: A translational platform technology. In Plasmonics in Biology and Medicine XI [895702] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8957). SPIE. https://doi.org/10.1117/12.2041115