Low concentrations of the soy phytoestrogen genistein induce proteinase inhibitor 9 and block killing of breast cancer cells by immune cells

Xinguo Jiang, Nicole M. Patterson, Yan Ling, Jianwei Xie, William G. Helferich, David J. Shapiro

Research output: Contribution to journalArticlepeer-review

Abstract

The risks and benefits of diets and supplements containing the estrogenic soy isoflavone genistein are not well established. We report that 10 nM genistein potently induces the granzyme B inhibitor, proteinase inhibitor 9 (PI-9) in MCF-7 human breast cancer cells. By inducing PI-9, genistein inhibits the ability of human natural killer (NK) cells to lyse the target breast cancer cells. In ERαHA cells, stably transfected MCF-7 cells, which contain elevated levels of estrogen receptor-α (ERα), 100 pM genistein or 17β-estradiol potently induce PI-9 and prevent NK cells from killing the target breast cancer cells. The concentrations of genistein that fully induce PI-9 in MCF-7 cells, and in ERαHA cells, are far lower than those previously reported to elicit estrogenic responses through ERα. Because 4-hydroxytamoxifen, raloxifene, and ICI 182,780/Faslodex all block genistein induction of PI-9 and elevated levels of ERα enhance induction of PI-9, genistein acts via ERα to induce PI-9. Increasing levels of ERα in breast cancer cells results in a progressive increase in induction of PI-9 by genistein and in the cell's ability to evade killing by NK cells. Moderate levels of dietary genistein and soy flour effectively induce PI-9 in human breast cancers grown in ovariectomized athymic mice. A significant population consumes levels of genistein in soy products that may be high enough to induce PI-9, perhaps potentiating the survival of some preexisting breast cancers by enabling them to evade immunosurveillance.

Original languageEnglish (US)
Pages (from-to)5366-5373
Number of pages8
JournalEndocrinology
Volume149
Issue number11
DOIs
StatePublished - Nov 2008

ASJC Scopus subject areas

  • Endocrinology

Fingerprint Dive into the research topics of 'Low concentrations of the soy phytoestrogen genistein induce proteinase inhibitor 9 and block killing of breast cancer cells by immune cells'. Together they form a unique fingerprint.

Cite this