Loss of cell adhesion in colon cancer cells during in vitro metastasis measured by bio-mems force sensor

Xin Tang, M Taher A Saif

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Human colon carcinoma (HCT-8) cells show metastatic phenotype when cultured on appropriately soft substrates. Here, we studied the surface non-specific adhesion in HCT-8 cells throughout the in vitro metastasis process. A novel bio-MEMS force sensor was used to measure the cell-probe nonspecific adhesion. The adhesion characteristics are analyzed using classical Johnson-Kendall- Roberts (JKR) theory. Our results indicate that the post-metastatic HCT-8 cells (dissociated R cells) display remarkably diminished surface adhesion and are potentially more invasive than original pre-metastatic HCT-8 cells (E cells). To the best of our knowledge, this is the first quantitative data on cancer cells adhesion change as in vitro metastasis proceeds.

Original languageEnglish (US)
Title of host publicationASME 2012 Summer Bioengineering Conference, SBC 2012
Pages251-252
Number of pages2
DOIs
StatePublished - Dec 1 2012
EventASME 2012 Summer Bioengineering Conference, SBC 2012 - Fajardo, Puerto Rico
Duration: Jun 20 2012Jun 23 2012

Publication series

NameASME 2012 Summer Bioengineering Conference, SBC 2012

Other

OtherASME 2012 Summer Bioengineering Conference, SBC 2012
CountryPuerto Rico
CityFajardo
Period6/20/126/23/12

Fingerprint

Cell adhesion
Adhesion
Cells
Sensors
MEMS
Substrates

ASJC Scopus subject areas

  • Bioengineering

Cite this

Tang, X., & Saif, M. T. A. (2012). Loss of cell adhesion in colon cancer cells during in vitro metastasis measured by bio-mems force sensor. In ASME 2012 Summer Bioengineering Conference, SBC 2012 (pp. 251-252). (ASME 2012 Summer Bioengineering Conference, SBC 2012). https://doi.org/10.1115/SBC2012-80936

Loss of cell adhesion in colon cancer cells during in vitro metastasis measured by bio-mems force sensor. / Tang, Xin; Saif, M Taher A.

ASME 2012 Summer Bioengineering Conference, SBC 2012. 2012. p. 251-252 (ASME 2012 Summer Bioengineering Conference, SBC 2012).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tang, X & Saif, MTA 2012, Loss of cell adhesion in colon cancer cells during in vitro metastasis measured by bio-mems force sensor. in ASME 2012 Summer Bioengineering Conference, SBC 2012. ASME 2012 Summer Bioengineering Conference, SBC 2012, pp. 251-252, ASME 2012 Summer Bioengineering Conference, SBC 2012, Fajardo, Puerto Rico, 6/20/12. https://doi.org/10.1115/SBC2012-80936
Tang X, Saif MTA. Loss of cell adhesion in colon cancer cells during in vitro metastasis measured by bio-mems force sensor. In ASME 2012 Summer Bioengineering Conference, SBC 2012. 2012. p. 251-252. (ASME 2012 Summer Bioengineering Conference, SBC 2012). https://doi.org/10.1115/SBC2012-80936
Tang, Xin ; Saif, M Taher A. / Loss of cell adhesion in colon cancer cells during in vitro metastasis measured by bio-mems force sensor. ASME 2012 Summer Bioengineering Conference, SBC 2012. 2012. pp. 251-252 (ASME 2012 Summer Bioengineering Conference, SBC 2012).
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