Long-term time-lapse multimodal microscopy for tracking cell dynamics in live tissue

Benedikt W. Graf, Maria C. Valero, Eric J. Chaney, Marina Marjanovic, Marni D. Boppart, Stephen A. Boppart

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

High speed intravital microscopy has emerged as an essential tool for studying cellular dynamics in live tissue. A limitation of this technique, however, is that the timescale that a sample can be continuously imaged is limited by practical considerations to several hours. Long term observation of live tissue is of great interest for a variety of research areas. We present methods for observing long term cellular dynamics in live tissue based on three-dimensional registration of time-lapse intravital microscopy images. For these experiments we utilized a custom multimodal microscope that allows simultaneous and co-registered acquisition of optical coherence (OCM) and multiphoton (MPM) microscopy images. OCM allows the structure of a sample to be visualized based on backscattered light while MPM excited fluorescence allows individual cells and cell function to be visualized. The OCM images of tissue structure are used to register data sets taken at different time points. The transformations of the OCM images are applied to MPM images to determine the migration of cell populations. This method of image registration is applied to in vivo tracking of bone-marrow derived GFP-labeled stem cells in mouse skin following bone marrow transplants from GFP donors into species-matched wildtype hosts. The use of three-dimensional image registration of time-lapse microscopy images enables tracking these cells after local cutaneous injury, and for investigating the role of skin stem cells in wound healing.

Original languageEnglish (US)
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
DOIs
StatePublished - Apr 20 2011
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX - San Francisco, CA, United States
Duration: Jan 22 2011Jan 25 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7902
ISSN (Print)1605-7422

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
CountryUnited States
CitySan Francisco, CA
Period1/22/111/25/11

Fingerprint

Cell Tracking
Microscopy
Microscopic examination
Tissue
microscopy
Image registration
Stem cells
cells
Skin
Bone
Stem Cells
Bone Marrow
Transplants
Three-Dimensional Imaging
bone marrow
stem cells
Wound Healing
Cell Movement
Microscopes
Fluorescence

Keywords

  • image registration
  • Intravital microscopy
  • multiphoton microscopy
  • optical coherence microscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Graf, B. W., Valero, M. C., Chaney, E. J., Marjanovic, M., Boppart, M. D., & Boppart, S. A. (2011). Long-term time-lapse multimodal microscopy for tracking cell dynamics in live tissue. In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX [790206] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7902). https://doi.org/10.1117/12.874183

Long-term time-lapse multimodal microscopy for tracking cell dynamics in live tissue. / Graf, Benedikt W.; Valero, Maria C.; Chaney, Eric J.; Marjanovic, Marina; Boppart, Marni D.; Boppart, Stephen A.

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX. 2011. 790206 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7902).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Graf, BW, Valero, MC, Chaney, EJ, Marjanovic, M, Boppart, MD & Boppart, SA 2011, Long-term time-lapse multimodal microscopy for tracking cell dynamics in live tissue. in Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX., 790206, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, San Francisco, CA, United States, 1/22/11. https://doi.org/10.1117/12.874183
Graf BW, Valero MC, Chaney EJ, Marjanovic M, Boppart MD, Boppart SA. Long-term time-lapse multimodal microscopy for tracking cell dynamics in live tissue. In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX. 2011. 790206. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.874183
Graf, Benedikt W. ; Valero, Maria C. ; Chaney, Eric J. ; Marjanovic, Marina ; Boppart, Marni D. ; Boppart, Stephen A. / Long-term time-lapse multimodal microscopy for tracking cell dynamics in live tissue. Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX. 2011. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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