Long-Range Directional Movement of an Interphase Chromosome Site

Chien Hui Chuang, Anne E. Carpenter, Beata Fuchsova, Terezina Johnson, Primal de Lanerolle, Andrew S Belmont

Research output: Contribution to journalArticle

Abstract

Increasing evidence suggests functional compartmentalization of interphase nuclei [1]. This includes preferential interior localization of gene-rich and early replicating chromosome regions versus peripheral localization of gene-poor and late replicating chromosome regions [2, 3], association of some active genes with nuclear speckles [4, 5] or transcription "factories" [6], and association of transcriptionally repressed genes with heterochromatic regions [7]. Dynamic changes in chromosome compartmentalization [7-9] imply mechanisms for long-range interphase chromatin movements. However, live cell imaging in mammalian cells has revealed limited chromatin mobility [10], described as "constrained diffusion" [11]. None of these studies, though, have examined a chromosome locus undergoing an inducible repositioning between two different nuclear compartments. Here we demonstrate migration of an interphase chromosome site from the nuclear periphery to the interior 1-2 hr after targeting a transcriptional activator to this site. Spot redistribution is perturbed by specific actin or nuclear myosin I mutants. Extended periods of chromosome immobility are interspersed with several minute periods in which chromosomes move unidirectionally along curvilinear paths oriented roughly perpendicular to the nuclear envelope at velocities of 0.1-0.9 μm/min over distances of 1-5 μm. Our results suggest an active mechanism for fast and directed long-range interphase chromosome movements dependent directly or indirectly on actin/myosin.

Original languageEnglish (US)
Pages (from-to)825-831
Number of pages7
JournalCurrent Biology
Volume16
Issue number8
DOIs
StatePublished - Apr 18 2006

Fingerprint

Interphase
Chromosomes
interphase
chromosomes
Genes
Chromatin
Actins
Myosin Type I
myosin
actin
chromatin
Chromosomes, Human, Pair 7
Chromosomes, Human, Pair 2
genes
Nuclear Envelope
Myosins
nuclear membrane
Transcription
Speckle
factories

Keywords

  • CELLBIO
  • DNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Chuang, C. H., Carpenter, A. E., Fuchsova, B., Johnson, T., de Lanerolle, P., & Belmont, A. S. (2006). Long-Range Directional Movement of an Interphase Chromosome Site. Current Biology, 16(8), 825-831. https://doi.org/10.1016/j.cub.2006.03.059

Long-Range Directional Movement of an Interphase Chromosome Site. / Chuang, Chien Hui; Carpenter, Anne E.; Fuchsova, Beata; Johnson, Terezina; de Lanerolle, Primal; Belmont, Andrew S.

In: Current Biology, Vol. 16, No. 8, 18.04.2006, p. 825-831.

Research output: Contribution to journalArticle

Chuang, CH, Carpenter, AE, Fuchsova, B, Johnson, T, de Lanerolle, P & Belmont, AS 2006, 'Long-Range Directional Movement of an Interphase Chromosome Site', Current Biology, vol. 16, no. 8, pp. 825-831. https://doi.org/10.1016/j.cub.2006.03.059
Chuang CH, Carpenter AE, Fuchsova B, Johnson T, de Lanerolle P, Belmont AS. Long-Range Directional Movement of an Interphase Chromosome Site. Current Biology. 2006 Apr 18;16(8):825-831. https://doi.org/10.1016/j.cub.2006.03.059
Chuang, Chien Hui ; Carpenter, Anne E. ; Fuchsova, Beata ; Johnson, Terezina ; de Lanerolle, Primal ; Belmont, Andrew S. / Long-Range Directional Movement of an Interphase Chromosome Site. In: Current Biology. 2006 ; Vol. 16, No. 8. pp. 825-831.
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