Localization and activity of Na+, K+ ‐ATPase in the ductuli efferentes of the rat

Kenneth Y. Ilio, Rex A. Hess

Research output: Contribution to journalArticle

Abstract

The Na+, K+ ‐ATPase enzyme through its p‐nitrophenyl phosphatase activity was localized in the ductuli efferentes of rats. Enzymatic activity was demonstrated along the cytoplasmic side of the plasmalemma of the ductular epithelial cells. The most intense deposition of reaction products was found on the plasmalemma delimiting the lower lateral and basal regions of the cells. The plasma membranes forming the microvilli, apical junctional complexes were devoid of reaction product while the midlateral membranes showed a weak reaction. The enzyme reaction was potassium‐dependent and was abolished by addition of 10 mM ouabain to the incubation media. Enzyme activity decreased significantly from proximal to distal regions of the ductules (8,101.47 ± 274.53, 6,658.95 ± 269.53 and 4,668.10 ± 575.41 pmoles p‐nitrophenol/mm/h, respectively in initial, conus vasculosus and terminal zones). A unified model for water absorption is proposed in the efferent ductules based upon this data and that of others.© Willey‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)190-200
Number of pages11
JournalThe Anatomical Record
Volume234
Issue number2
DOIs
StatePublished - Oct 1992

Keywords

  • Cytochemistry
  • Efferent ductules
  • Histochemistry
  • Water and ion transport

ASJC Scopus subject areas

  • Anatomy
  • Agricultural and Biological Sciences (miscellaneous)

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