TY - JOUR
T1 - Local environment of surface-polyelectrolyte-bound DNA oligomers
AU - Jeon, Sangmin
AU - Bae, Sung Chul
AU - Zhao, Jiang John
AU - Granick, Steve
N1 - Funding Information:
This work was supported by the National Science Foundation and by the U.S. Department of Energy, Division of Materials Science through the Frederick Seitz Materials Research Laboratory at the University of Illinois at Urbana-Champaign.
PY - 2001
Y1 - 2001
N2 - Two-photon time-resolved fluorescence anisotropy methods were used to study the dynamical environment when fluorescent-labelled DNA oligomers (labelled with FAM, 6-fluorescein-6-carboxamido hexanoate) formed surface complexes with quaternized polyvinylpyridine (QPVP) cationic layers on a glass surface. We compared the anisotropy decay of DNA in bulk aqueous solution, DNA adsorbed onto QPVP, and QPVP-DNA-QPVP sandwich structures. When DNA was adsorbed onto QPVP, its anisotropy decay was dramatically retarded compared to the bulk, which means it had very slow rotational motion on the surface. Motions slowed down with increasing salt concentration up to a level of 0.1 M NaCl, but mobility began to increase at still higher salt concentration owing to detachment from the surface-immobilizing QPVP layers.
AB - Two-photon time-resolved fluorescence anisotropy methods were used to study the dynamical environment when fluorescent-labelled DNA oligomers (labelled with FAM, 6-fluorescein-6-carboxamido hexanoate) formed surface complexes with quaternized polyvinylpyridine (QPVP) cationic layers on a glass surface. We compared the anisotropy decay of DNA in bulk aqueous solution, DNA adsorbed onto QPVP, and QPVP-DNA-QPVP sandwich structures. When DNA was adsorbed onto QPVP, its anisotropy decay was dramatically retarded compared to the bulk, which means it had very slow rotational motion on the surface. Motions slowed down with increasing salt concentration up to a level of 0.1 M NaCl, but mobility began to increase at still higher salt concentration owing to detachment from the surface-immobilizing QPVP layers.
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M3 - Conference article
AN - SCOPUS:0035559606
SN - 0272-9172
VL - 651
SP - T4.2b.1-T4.2b.8
JO - Materials Research Society Symposium - Proceedings
JF - Materials Research Society Symposium - Proceedings
T2 - Dynamics in Small Confining Systems V
Y2 - 27 November 2000 through 30 November 2000
ER -