Local environment of surface-polyelectrolyte-bound DNA oligomers

Sangmin Jeon, Sung Chul Bae, Jiang John Zhao, Steve Granick

Research output: Contribution to journalConference article

Abstract

Two-photon time-resolved fluorescence anisotropy methods were used to study the dynamical environment when fluorescent-labelled DNA oligomers (labelled with FAM, 6-fluorescein-6-carboxamido hexanoate) formed surface complexes with quaternized polyvinylpyridine (QPVP) cationic layers on a glass surface. We compared the anisotropy decay of DNA in bulk aqueous solution, DNA adsorbed onto QPVP, and QPVP-DNA-QPVP sandwich structures. When DNA was adsorbed onto QPVP, its anisotropy decay was dramatically retarded compared to the bulk, which means it had very slow rotational motion on the surface. Motions slowed down with increasing salt concentration up to a level of 0.1 M NaCl, but mobility began to increase at still higher salt concentration owing to detachment from the surface-immobilizing QPVP layers.

Original languageEnglish (US)
Pages (from-to)T4.2b.1-T4.2b.8
JournalMaterials Research Society Symposium - Proceedings
Volume651
StatePublished - Dec 1 2001
EventDynamics in Small Confining Systems V - Boston, MA, United States
Duration: Nov 27 2000Nov 30 2000

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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    Jeon, S., Bae, S. C., Zhao, J. J., & Granick, S. (2001). Local environment of surface-polyelectrolyte-bound DNA oligomers. Materials Research Society Symposium - Proceedings, 651, T4.2b.1-T4.2b.8.