Live cell refractometry using microfluidic devices

Niyom Lue; Gabriel Popescu; Takahiro Ikeda; Ramachandra R. Dasari; Kamran Badizadegan; Michael S. Feld

doi:10.1364/OL.31.002759

Live cell refractometry using microfluidic devices

Niyom Lue, Gabriel Popescu, Takahiro Ikeda, Ramachandra R. Dasari, Kamran Badizadegan, Michael S. Feld

Research output: Contribution to journal › Article › peer-review

Abstract

Using Hilbert phase microscopy for extracting quantitative phase images, we measured the average refractive index associated with live cells in culture. Tb decouple the contributions to the phase signal from the cell refractive index and thickness, we confined the cells in microchannels. The results are confirmed by comparison with measurements of spherical cells in suspension.

Original language	English (US)
Pages (from-to)	2759-2761
Number of pages	3
Journal	Optics Letters
Volume	31
Issue number	18
DOIs	https://doi.org/10.1364/OL.31.002759
State	Published - Sep 15 2006
Externally published	Yes

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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10.1364/OL.31.002759

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title = "Live cell refractometry using microfluidic devices",

abstract = "Using Hilbert phase microscopy for extracting quantitative phase images, we measured the average refractive index associated with live cells in culture. Tb decouple the contributions to the phase signal from the cell refractive index and thickness, we confined the cells in microchannels. The results are confirmed by comparison with measurements of spherical cells in suspension.",

author = "Niyom Lue and Gabriel Popescu and Takahiro Ikeda and Dasari, {Ramachandra R.} and Kamran Badizadegan and Feld, {Michael S.}",

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AU - Popescu, Gabriel

AU - Ikeda, Takahiro

AU - Dasari, Ramachandra R.

AU - Badizadegan, Kamran

AU - Feld, Michael S.

PY - 2006/9/15

Y1 - 2006/9/15

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AB - Using Hilbert phase microscopy for extracting quantitative phase images, we measured the average refractive index associated with live cells in culture. Tb decouple the contributions to the phase signal from the cell refractive index and thickness, we confined the cells in microchannels. The results are confirmed by comparison with measurements of spherical cells in suspension.

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Live cell refractometry using microfluidic devices

Abstract

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