Lipid mobility in Acholeplasma membranes using deuteron magnetic resonance

E. Oldfield, D. Chapman, W. Derbyshire

Research output: Contribution to journalArticle

Abstract

Acholeplasma laidlawii B have been grown on media supplemented with perdeuterated lauric and palmitic acids. The lauric acid was shown to be elongated to myristic acid-d23 and palmitic acid-d23, and these acids were incorporated into the membrane lipids. Infrared measurements indicated the membrane proteins were predominantly α-helical. Deuteron magnetic resonance spectroscopy of the deuterated lipids in intact membranes indicated that the lipids were in a state of mobility rather similar to that of the gel state of dimyristoyl lecithin. This is consistent with our interpretation of results obtained by differential scanning calorimetry and X-ray diffraction. The existence of rigid lipid regions in organisms lacking cholesterol may not be uncommon, and may occur in E. coli also.

Original languageEnglish (US)
Pages (from-to)69-81
Number of pages13
JournalChemistry and Physics of Lipids
Volume9
Issue number1
DOIs
StatePublished - Jul 1972
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Cell Biology

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