Linoleic and α-linolenic fatty acid consumption over three generations exert cumulative regulation of hepatic expression of genes related to lipid metabolism

Carolina B. Jacometo; Eduardo Schmitt; Luiz F.M. Pfeifer; Augusto Schneider; Francielle Bado; Fernanda T. Da Rosa; Simone Halfen; Francisco A.B. Del Pino; Juan J. Loor; Marcio N. Corrêa; Nelson J.L. Dionello

doi:10.1007/s12263-014-0405-7

Linoleic and α-linolenic fatty acid consumption over three generations exert cumulative regulation of hepatic expression of genes related to lipid metabolism

Carolina B. Jacometo, Eduardo Schmitt, Luiz F.M. Pfeifer, Augusto Schneider, Francielle Bado, Fernanda T. Da Rosa, Simone Halfen, Francisco A.B. Del Pino, Juan J. Loor, Marcio N. Corrêa, Nelson J.L. Dionello

Research output: Contribution to journal › Article › peer-review

Abstract

The essential fatty acids, omega-3 and omega-6, consumed during pregnancy can benefit maternal and offspring health. For instance, they could activate a network of genes related to the nuclear receptor peroxisome proliferator- activated receptor α (Ppara) and sterol regulatory element binding transcription factor 1 (Srebf1), which play a role in fatty acid oxidation and lipogenesis. The present study aimed to investigate the effects of diets with different omega-3/omega-6 ratio consumed over three generations on blood biochemical parameters and hepatic expression of Ppara- and Srebf1-related genes. During three consecutive generations adult Wistar rats were evaluated in the postpartum period (21 days after parturition). Regardless of prenatal dietary omega-3/omega-6 ratio, an upregulation in liver tissue was observed for Rxra, Lxra and Srebf1 and a downregulation for Fasn in all the evaluated generations. The diet with higher omega-3/omega-6 ratio decreased triacylglycerol serum levels and resulted in a constant non-esterified fatty acid level. Our results indicated that the PUFAs effect on the modulation of genes related to fatty acid oxidation and lipogenesis is cumulative through generations.

Original language	English (US)
Article number	405
Journal	Genes and Nutrition
Volume	9
Issue number	4
DOIs	https://doi.org/10.1007/s12263-014-0405-7
State	Published - Jul 2014

Keywords

Fasn
Lxra
PUFAs
Ppara
Rxra
Srebf1

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Genetics

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10.1007/s12263-014-0405-7

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Jacometo, C. B., Schmitt, E., Pfeifer, L. F. M., Schneider, A., Bado, F., Da Rosa, F. T., Halfen, S., Del Pino, F. A. B., Loor, J. J., Corrêa, M. N., & Dionello, N. J. L. (2014). Linoleic and α-linolenic fatty acid consumption over three generations exert cumulative regulation of hepatic expression of genes related to lipid metabolism. Genes and Nutrition, 9(4), Article 405. https://doi.org/10.1007/s12263-014-0405-7

Jacometo, CB, Schmitt, E, Pfeifer, LFM, Schneider, A, Bado, F, Da Rosa, FT, Halfen, S, Del Pino, FAB, Loor, JJ, Corrêa, MN & Dionello, NJL 2014, 'Linoleic and α-linolenic fatty acid consumption over three generations exert cumulative regulation of hepatic expression of genes related to lipid metabolism', Genes and Nutrition, vol. 9, no. 4, 405. https://doi.org/10.1007/s12263-014-0405-7

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title = "Linoleic and α-linolenic fatty acid consumption over three generations exert cumulative regulation of hepatic expression of genes related to lipid metabolism",

abstract = "The essential fatty acids, omega-3 and omega-6, consumed during pregnancy can benefit maternal and offspring health. For instance, they could activate a network of genes related to the nuclear receptor peroxisome proliferator- activated receptor α (Ppara) and sterol regulatory element binding transcription factor 1 (Srebf1), which play a role in fatty acid oxidation and lipogenesis. The present study aimed to investigate the effects of diets with different omega-3/omega-6 ratio consumed over three generations on blood biochemical parameters and hepatic expression of Ppara- and Srebf1-related genes. During three consecutive generations adult Wistar rats were evaluated in the postpartum period (21 days after parturition). Regardless of prenatal dietary omega-3/omega-6 ratio, an upregulation in liver tissue was observed for Rxra, Lxra and Srebf1 and a downregulation for Fasn in all the evaluated generations. The diet with higher omega-3/omega-6 ratio decreased triacylglycerol serum levels and resulted in a constant non-esterified fatty acid level. Our results indicated that the PUFAs effect on the modulation of genes related to fatty acid oxidation and lipogenesis is cumulative through generations.",

keywords = "Fasn, Lxra, PUFAs, Ppara, Rxra, Srebf1",

author = "Jacometo, {Carolina B.} and Eduardo Schmitt and Pfeifer, {Luiz F.M.} and Augusto Schneider and Francielle Bado and {Da Rosa}, {Fernanda T.} and Simone Halfen and {Del Pino}, {Francisco A.B.} and Loor, {Juan J.} and Corr{\^e}a, {Marcio N.} and Dionello, {Nelson J.L.}",

note = "Funding Information: Acknowledgments We thank Dra. Leonor Almeida de Souza Soares for help with the experimental diets and animal handling training. This research was funded by the PNPD Program (2278/2009) from the Coordination for the Improvement of Higher Level-or Education-Personnel (CAPES).",

year = "2014",

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doi = "10.1007/s12263-014-0405-7",

language = "English (US)",

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T1 - Linoleic and α-linolenic fatty acid consumption over three generations exert cumulative regulation of hepatic expression of genes related to lipid metabolism

AU - Jacometo, Carolina B.

AU - Schmitt, Eduardo

AU - Pfeifer, Luiz F.M.

AU - Schneider, Augusto

AU - Bado, Francielle

AU - Da Rosa, Fernanda T.

AU - Halfen, Simone

AU - Del Pino, Francisco A.B.

AU - Loor, Juan J.

AU - Corrêa, Marcio N.

AU - Dionello, Nelson J.L.

N1 - Funding Information: Acknowledgments We thank Dra. Leonor Almeida de Souza Soares for help with the experimental diets and animal handling training. This research was funded by the PNPD Program (2278/2009) from the Coordination for the Improvement of Higher Level-or Education-Personnel (CAPES).

PY - 2014/7

Y1 - 2014/7

N2 - The essential fatty acids, omega-3 and omega-6, consumed during pregnancy can benefit maternal and offspring health. For instance, they could activate a network of genes related to the nuclear receptor peroxisome proliferator- activated receptor α (Ppara) and sterol regulatory element binding transcription factor 1 (Srebf1), which play a role in fatty acid oxidation and lipogenesis. The present study aimed to investigate the effects of diets with different omega-3/omega-6 ratio consumed over three generations on blood biochemical parameters and hepatic expression of Ppara- and Srebf1-related genes. During three consecutive generations adult Wistar rats were evaluated in the postpartum period (21 days after parturition). Regardless of prenatal dietary omega-3/omega-6 ratio, an upregulation in liver tissue was observed for Rxra, Lxra and Srebf1 and a downregulation for Fasn in all the evaluated generations. The diet with higher omega-3/omega-6 ratio decreased triacylglycerol serum levels and resulted in a constant non-esterified fatty acid level. Our results indicated that the PUFAs effect on the modulation of genes related to fatty acid oxidation and lipogenesis is cumulative through generations.

AB - The essential fatty acids, omega-3 and omega-6, consumed during pregnancy can benefit maternal and offspring health. For instance, they could activate a network of genes related to the nuclear receptor peroxisome proliferator- activated receptor α (Ppara) and sterol regulatory element binding transcription factor 1 (Srebf1), which play a role in fatty acid oxidation and lipogenesis. The present study aimed to investigate the effects of diets with different omega-3/omega-6 ratio consumed over three generations on blood biochemical parameters and hepatic expression of Ppara- and Srebf1-related genes. During three consecutive generations adult Wistar rats were evaluated in the postpartum period (21 days after parturition). Regardless of prenatal dietary omega-3/omega-6 ratio, an upregulation in liver tissue was observed for Rxra, Lxra and Srebf1 and a downregulation for Fasn in all the evaluated generations. The diet with higher omega-3/omega-6 ratio decreased triacylglycerol serum levels and resulted in a constant non-esterified fatty acid level. Our results indicated that the PUFAs effect on the modulation of genes related to fatty acid oxidation and lipogenesis is cumulative through generations.

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KW - Rxra

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ER -

Linoleic and α-linolenic fatty acid consumption over three generations exert cumulative regulation of hepatic expression of genes related to lipid metabolism

Abstract

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