Lineage-specific biology revealed by a finished genome assembly of the mouse

Deanna M. Church, Leo Goodstadt, Ladeana W. Hillier, Michael C. Zody, Steve Goldstein, Xinwe She, Carol J. Bult, Richa Agarwala, Joshua L. Cherry, Michael DiCuccio, Wratko Hlavina, Yuri Kapustin, Peter Meric, Donna Maglott, Zoë Birtle, Ana C. Marques, Tina Graves, Shiguo Zhou, Brian Teague, Konstantinos PotamousisChristopher Churas, Michael Place, Jill Herschleb, Ron Runnheim, Daniel Forrest, James Amos-Landgraf, David C. Schwartz, Ze Cheng, Kerstin Lindblad-Toh, Evan E. Eichler, Chris P. Ponting, Donna M. Muzny, Shannon Dugan-Rocha, Yan Ding, Steven E. Scherer, Christian J. Buhay, Andrew Cree, Judith Hernandez, Michael Holder, Jennifer Hume, Laronda R. Jackson, Christie Kovar, Sandra L. Lee, Lora R. Lewis, Michael L. Metzker, Lynne V. Narareth, Aniko Sabo, Erica Sodergren, Richard A. Gibbs, Michael FitzGerald, April Cook, David B. Jaffe, Manuel Garber, Andrew R. Zimmer, Mono Pirun, Lyndsey Russell, Ted Sharpe, Michael Kamal Kabir Chaturvedi, Jane Wilkinson, Kurt LaButti, Xiaoping Yang, Daniel Bessette, Nicole R. Allen, Cindy Nguyen, Thu Nguyen, Chelsea Dunbar, Rakela Lubonja, Charles Matthews, Xiaohong Liu, Mostafa Benamara, Tamrat Negash, Tashi Lokyitsang, Karin Decktor, Bruno Piqani, Glen Munson, Pema Tenzin, Sabrina Stone, Pendexter Macdonald, Harindra Arachchi, Amr Abouelleil, Annie Lui, Margaret Priest, Gary Gearin, Adam Brown, Lynne Aftuck, Terrance Shea, Sean Sykes, Aaron Berlin, Jeff Chu, Kathleen Dooley, Daniel Hagopian, Jennifer Hall, Nabil Hafez, Cherylyn L. Smith, Peter Olandt, Karen Miller, Vijay Ventkataraman, Anthony Rachupka, Lester Dorris, Laura Ayotte, Richard Mabbitt, Jeffrey Erickson, Andrea Horn, Peter An, Jerome W. Naylor, Sampath Settipalli, Eric S. Lander, Richard K. Wilson, Tina A. Graves, Robert S. Fulton, Susan M. Rock, Asif T. Chinwalla, Kelly Bernard, Laura P. Courtney, Catrina Fronick, Lucinda L. Fulton, Michelle O'Laughlin, Colin L. Kremitzki, Patrick J. Minx, Joanne O. Nelson, Kyriena L. Schatzkamer, Cynthia Strong, Aye M. Wollam, George M. Weinstock, Shiaw Pyng Yang, Jane Rogers, Darren Grafham, Sean Humphray, Christine Nicholson, Christine Bird, Andrew J. Brown, John Burton, Chris Clee, Adrienne Hunt, Matt C. Jones, Christine Lloyd, Lucy Matthews, Karen Mclaren, Stuart Mclaren, Kirsten McLay, Sophie A. Palmer, Robert Plumb, Ratna Shownkeen, Sarah Sims, Mike A. Quail, Siobhan L. Whitehead, David L. Willey, Stephane Deschamps, Steven Kenton, Lin Song, Trang Do, Bruce Roe, Gerard G. Bouffard, Robert W. Blakesley, Eric D. Green, Raju Kucherlapati, George Grills, Li Li, Kate T. Montgomery, Melissa Kramer, Lori Speigel, W. Richard McCombie, Susan Lucas, Astrid Terry, Laurie Gordon, Lisa Stubbs, Paul Denny, Steve D.M. Brown, Anne Marie Mallon, R. Duncan Campbell, Marc R.M. Botherby, Ian J. Jackson, Marc J. Rubenfield, Andrea M. Rogosin, Douglas R. Smith

Research output: Contribution to journalArticle

Abstract

The mouse (Mus musculus) is the premier animal model for understanding human disease and development. Here we show that a comprehensive understanding of mouse biology is only possible with the availability of a finished, high-quality genome assembly. The finished clone-based assembly of the mouse strain C57BL/6J reported here has over 175,000 fewer gaps and over 139 Mb more of novel sequence, compared with the earlier MGSCv3 draft genome assembly. In a comprehensive analysis of this revised genome sequence, we are now able to define 20,210 protein-coding genes, over a thousand more than predicted in the human genome (19,042 genes). In addition, we identified 439 long, non-proteincoding RNAs with evidence for transcribed orthologs in human. We analyzed the complex and repetitive landscape of 267 Mb of sequence that was missing or misassembled in the previously published assembly, and we provide insights into the reasons for its resistance to sequencing and assembly by whole-genome shotgun approaches. Duplicated regions within newly assembled sequence tend to be of more recent ancestry than duplicates in the published draft, correcting our initial understanding of recent evolution on the mouse lineage. These duplicates appear to be largely composed of sequence regions containing transposable elements and duplicated protein-coding genes; of these, some may be fixed in the mouse population, but at least 40% of segmentally duplicated sequences are copy number variable even among laboratory mouse strains. Mouse lineage-specific regions contain 3,767 genes drawn mainly from rapidly-changing gene families associated with reproductive functions. The finished mouse genome assembly, therefore, greatly improves our understanding of rodent-specific biology and allows the delineation of ancestral biological functions that are shared with human from derived functions that are not.

Original languageEnglish (US)
Article numbere1000112
JournalPLoS biology
Volume7
Issue number5
DOIs
StatePublished - May 2009

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

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    Church, D. M., Goodstadt, L., Hillier, L. W., Zody, M. C., Goldstein, S., She, X., Bult, C. J., Agarwala, R., Cherry, J. L., DiCuccio, M., Hlavina, W., Kapustin, Y., Meric, P., Maglott, D., Birtle, Z., Marques, A. C., Graves, T., Zhou, S., Teague, B., ... Smith, D. R. (2009). Lineage-specific biology revealed by a finished genome assembly of the mouse. PLoS biology, 7(5), [e1000112]. https://doi.org/10.1371/journal.pbio.1000112