TY - JOUR
T1 - Lignocellulosic hydrolysates and extracellular electron shuttles for H2 production using co-culture fermentation with Clostridium beijerinckii and Geobacter metallireducens
AU - Zhang, Xinyu
AU - Ye, Xiaofeng
AU - Guo, Bin
AU - Finneran, Kevin T.
AU - Zilles, Julie L.
AU - Morgenroth, Eberhard
N1 - Funding Information:
We thank Hans Blaschek and Steven Stoddard of TetraVitae Biosciences for Clostridium beijerinckii spores and Derek R. Lovley of the University of Massachusetts for Geobacter metallireducens . This work was supported by National Science Foundation grant No. 0756054 .
PY - 2013/11
Y1 - 2013/11
N2 - A co-culture of Clostridium beijerinckii and Geobacter metallireducens with AH2QDS produced hydrogen from lignocellulosic hydrolysates (biomass of Miscanthus prepared by hydrothermal treatment with dilute acids). This co-culture system enhanced hydrogen production from lignocellulosic hydrolysates by improving substrate utilization and diminishing acetate accumulation, despite the presence of fermentation inhibitors in the hydrolysates. The improvements were greater for xylose-rich hydrolysates. The increase in maximum cumulative hydrogen production for hydrolysates with glucose:xylose mass ratios of 1:0.2, 1:1 and 1:10g/g was 0%, 22% and 11%, respectively. Alternative extracellular electron shuttles (EES), including indigo dye, juglone, lawsone, fulvic acids and humic acids, were able to substitute for AH2QDS, improving hydrogen production in the co-culture system using xylose as model substrate. Increased utilization of xylose-rich hydrolysates and substitution of alternative EES make the co-culture with EES system a more attractive strategy for industrial biohydrogen production.
AB - A co-culture of Clostridium beijerinckii and Geobacter metallireducens with AH2QDS produced hydrogen from lignocellulosic hydrolysates (biomass of Miscanthus prepared by hydrothermal treatment with dilute acids). This co-culture system enhanced hydrogen production from lignocellulosic hydrolysates by improving substrate utilization and diminishing acetate accumulation, despite the presence of fermentation inhibitors in the hydrolysates. The improvements were greater for xylose-rich hydrolysates. The increase in maximum cumulative hydrogen production for hydrolysates with glucose:xylose mass ratios of 1:0.2, 1:1 and 1:10g/g was 0%, 22% and 11%, respectively. Alternative extracellular electron shuttles (EES), including indigo dye, juglone, lawsone, fulvic acids and humic acids, were able to substitute for AH2QDS, improving hydrogen production in the co-culture system using xylose as model substrate. Increased utilization of xylose-rich hydrolysates and substitution of alternative EES make the co-culture with EES system a more attractive strategy for industrial biohydrogen production.
KW - Co-culture
KW - Extracellular electron shuttles
KW - Hydrogen
KW - Lignocellulose
KW - Xylose
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U2 - 10.1016/j.biortech.2013.07.106
DO - 10.1016/j.biortech.2013.07.106
M3 - Article
C2 - 23994308
AN - SCOPUS:84883250852
SN - 0960-8524
VL - 147
SP - 89
EP - 95
JO - Bioresource Technology
JF - Bioresource Technology
ER -