Light scattering of human red blood cells during metabolic remodeling of the membrane

Yongkeun Park; Catherine A. Best-Popescu; Ramachandra R. Dasari; Gabriel Popescu

doi:10.1117/1.3524509

Light scattering of human red blood cells during metabolic remodeling of the membrane

Yongkeun Park, Catherine A. Best-Popescu, Ramachandra R. Dasari, Gabriel Popescu

Research output: Contribution to journal › Article › peer-review

Abstract

We present the light scattering properties of individual human red blood cells (RBCs). We show that both the RBC static and dynamic scattering signals are altered by adenosine 5'-triphosphate (ATP)-driven membrane metabolic remodeling. To measure the light scattering signal from individual RBCs, we use diffraction phase microscopy together with a Fourier transform light scattering technique. RBC cytosolic ATPs are both chemically and metabolically depleted, and the corresponding scattering signals are compared with the light scattering signal of normal RBCs having physiologic levels of ATP.

Original language	English (US)
Article number	011013
Journal	Journal of biomedical optics
Volume	16
Issue number	1
DOIs	https://doi.org/10.1117/1.3524509
State	Published - Jan 2011

Keywords

erythrocyte
light scattering
quantitative phase microscopy
red blood cells

ASJC Scopus subject areas

Biomedical Engineering
Biomaterials
Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics

Online availability

10.1117/1.3524509

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abstract = "We present the light scattering properties of individual human red blood cells (RBCs). We show that both the RBC static and dynamic scattering signals are altered by adenosine 5'-triphosphate (ATP)-driven membrane metabolic remodeling. To measure the light scattering signal from individual RBCs, we use diffraction phase microscopy together with a Fourier transform light scattering technique. RBC cytosolic ATPs are both chemically and metabolically depleted, and the corresponding scattering signals are compared with the light scattering signal of normal RBCs having physiologic levels of ATP.",

keywords = "erythrocyte, light scattering, quantitative phase microscopy, red blood cells",

author = "Yongkeun Park and Best-Popescu, {Catherine A.} and Dasari, {Ramachandra R.} and Gabriel Popescu",

note = "Funding Information: This research was supported by the National Institutes of Health (P41-RR02594–18-24). Author Park was supported by Sam-sung Scholarship. Author Popescu acknowledges support from the National Science Foundation (CAREER: 08–46660) and National Cancer Institute (R21: CA154813). The authors appreciate the guidance and advice from the late Michael S. Feld.",

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Light scattering of human red blood cells during metabolic remodeling of the membrane

Abstract

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