Light-responsive helical polypeptides capable of reducing toxicity and unpacking DNA: Toward nonviral gene delivery

Lichen Yin, Haoyu Tang, Kyung Hoon Kim, Nan Zheng, Ziyuan Song, Nathan P. Gabrielson, Hua Lu, Jianjun Cheng

Research output: Contribution to journalArticlepeer-review

Abstract

Gene delivery vehicles: Helical, cationic polypeptides with light-responsive domains showed potent membrane activity and promoted efficient cellular internalization of DNA (see picture). After transfection, a light trigger induces protecting-group removal to expose anionic carboxylate groups. The reduced cationic charge and helix distortion of the polypeptides result in enhanced DNA unpacking and reduced material toxicity because of the eliminated membrane activity.

Original languageEnglish (US)
Pages (from-to)9182-9186
Number of pages5
JournalAngewandte Chemie - International Edition
Volume52
Issue number35
DOIs
StatePublished - Aug 26 2013

Keywords

  • cell-penetrating peptides
  • charge conversion
  • helical structures
  • nonviral gene delivery

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

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