Abstract

The small sample volumes used in capillary electrophoresis (CE) make the technique suitable for myriad small-scale biochemical and materials science applications. These small volumes also require detection methods that exhibit high sensitivity and suitable selectivity. Such rigorous demands on detector performance can have important consequences with respect to other aspects of the CE system. For example, it is well known that higher efficiency and faster separations are achieved using smaller inner diameter (ID) capillaries. And yet 50-µm ID capillaries are more common than 5-µm ID capillaries in systems using light-based detection, primarily because the longer optical path length of the larger capillary aids in detection. In this way, the performance of the detection system can dictate the overall performance of a given CE system. Thus, knowledge of the various detection modes available, as well as their respective figures of merit, aids in designing CE-based methods of analysis (see Table 9.1). 1 .

Original languageEnglish (US)
Title of host publicationHandbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition
PublisherCRC Press
Pages305-334
Number of pages30
ISBN (Electronic)9781420004953
ISBN (Print)9780849333293
StatePublished - Jan 1 2007

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
Light
performance
Materials science
Detectors
efficiency
science

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Social Sciences(all)
  • Chemistry(all)

Cite this

Scanlan, C., Lapainis, T., & Sweedler, J. V. (2007). Light-based detection methods for capillary electrophoresis. In Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition (pp. 305-334). CRC Press.

Light-based detection methods for capillary electrophoresis. / Scanlan, Cory; Lapainis, Theodore; Sweedler, Jonathan V.

Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition. CRC Press, 2007. p. 305-334.

Research output: Chapter in Book/Report/Conference proceedingChapter

Scanlan, C, Lapainis, T & Sweedler, JV 2007, Light-based detection methods for capillary electrophoresis. in Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition. CRC Press, pp. 305-334.
Scanlan C, Lapainis T, Sweedler JV. Light-based detection methods for capillary electrophoresis. In Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition. CRC Press. 2007. p. 305-334
Scanlan, Cory ; Lapainis, Theodore ; Sweedler, Jonathan V. / Light-based detection methods for capillary electrophoresis. Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition. CRC Press, 2007. pp. 305-334
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