Lentivirus-mediated RNAi in skeletal myogenesis

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

RNA interference (RNAi) has greatly facilitated investigation of gene functions in vitro as well as in vivo. Recombinant lentivirus is widely used to deliver small hairpin RNA (shRNA) because of its high transduction capacity into diverse cell types and tissues. Here, we describe methods of lentivirus-mediated delivery of shRNA for the study of skeletal muscle cell differentiation in vitro and injury-induced muscle regeneration in mice.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages95-110
Number of pages16
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1889
ISSN (Print)1064-3745

Keywords

  • Gene knockdown
  • Lentivirus
  • Muscle regeneration
  • RNAi
  • Skeletal myogenesis
  • shRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Lentivirus-mediated RNAi in skeletal myogenesis'. Together they form a unique fingerprint.

Cite this