Lentivirus-mediated RNAi in skeletal myogenesis

Dongwook Kim, Adriana Reyes-Ordoñez, Jie Chen

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

RNA interference (RNAi) has greatly facilitated investigation of gene functions in vitro as well as in vivo. Recombinant lentivirus is widely used to deliver small hairpin RNA (shRNA) because of its high transduction capacity into diverse cell types and tissues. Here, we describe methods of lentivirus-mediated delivery of shRNA for the study of skeletal muscle cell differentiation in vitro and injury-induced muscle regeneration in mice.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages95-110
Number of pages16
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1889
ISSN (Print)1064-3745

Fingerprint

Lentivirus
Muscle Development
RNA Interference
Small Interfering RNA
Muscle Cells
Regeneration
Cell Differentiation
Skeletal Muscle
Muscles
Wounds and Injuries
Genes
In Vitro Techniques

Keywords

  • Gene knockdown
  • Lentivirus
  • Muscle regeneration
  • RNAi
  • shRNA
  • Skeletal myogenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Kim, D., Reyes-Ordoñez, A., & Chen, J. (2019). Lentivirus-mediated RNAi in skeletal myogenesis. In Methods in Molecular Biology (pp. 95-110). (Methods in Molecular Biology; Vol. 1889). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8897-6_7

Lentivirus-mediated RNAi in skeletal myogenesis. / Kim, Dongwook; Reyes-Ordoñez, Adriana; Chen, Jie.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 95-110 (Methods in Molecular Biology; Vol. 1889).

Research output: Chapter in Book/Report/Conference proceedingChapter

Kim, D, Reyes-Ordoñez, A & Chen, J 2019, Lentivirus-mediated RNAi in skeletal myogenesis. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1889, Humana Press Inc., pp. 95-110. https://doi.org/10.1007/978-1-4939-8897-6_7
Kim D, Reyes-Ordoñez A, Chen J. Lentivirus-mediated RNAi in skeletal myogenesis. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 95-110. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8897-6_7
Kim, Dongwook ; Reyes-Ordoñez, Adriana ; Chen, Jie. / Lentivirus-mediated RNAi in skeletal myogenesis. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 95-110 (Methods in Molecular Biology).
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